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|Tested species reactivity||Avian , Fish , Human , Hamster , Mouse , Primate , Porcine , Rabbit , Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the N-terminus of Human beta-Actin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris citrate/tris phosphate, pH 7-8|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Blocking Assay (BLOCK)||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:100-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is expected to cross-react with a wide range of species based on sequence homology.
Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
actin, beta; actin, cytoplasmic 1; Atcb; beta cytoskeletal actin; beta-actin; cytoplasmic beta-actin; gamma non-muscle actin; PS1TP5-binding protein 1; PS1TP5BP1
ACT-5; ACTB; Actx; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1