Proteintech

beta Actin Monoclonal Antibody (7D2C10), HRP

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beta Actin Antibody in Western Blot (WB) — Western blot analysis of beta actin in various tissues and cell lines using Proteintech antibody HRP-60008 at a dilution of 1:5000.

Product Details

HRP-60008

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000-1:16,000	View 26 publications 26 publications
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Plant, Rat, Zebrafish
Published species	Hamster, Human, Mouse, Rat
Host/Isotype	Mouse / IgM
Class	Monoclonal
Type	Antibody
Clone	7D2C10
Immunogen	Recombinant protein
Conjugate	HRP Biotin CoraLite488 CoraLite594
Form	Liquid
Concentration	0.33 mg/mL
Purification	Caprylic acid precipitation
Storage buffer	PBS, pH 7.3, with 50% glycerol, 0.5% BSA
Contains	0.02% thimerosal, 0.05% ProClin 300
Storage conditions	-20° C, store in dark

Product Specific Information

This antibody can recognize all of actins. This antibody is conjugated with HRP. Note: Do not add azide (Sodium Azide or Smite) into the dilution buffer. azide is the HRP inhibitor which decreases the enzymatic activity of HRP.

Target Information

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Isoliquiritigenin attenuates LPS-induced AKI by suppression of inflammation involving NF-κB pathway.

American journal of translational research

Tang Y,Wang C,Wang Y,Zhang J,Wang F,Li L,Meng X,Li G,Li Y,Wang L

Wed Sep 30 00:00:00 EDT 2020

Development of an Infectious Cell Culture System for Hepatitis C Virus Genotype 6a Clinical Isolate Using a Novel Strategy and Its Sensitivity to Direct-Acting Antivirals.

Frontiers in microbiology

Chen M,Zheng F,Yuan G,Duan X,Rong L,Liu J,Feng S,Wang Z,Wang M,Feng Y,Zhou Q,Li J,Deng K,Li C,Xia J,Rao G,Zhou Y,Fu Y,Li YP

Tue Sep 29 00:00:00 EDT 2020

G-1 exhibit antidepressant effect, increase of hippocampal ERs expression and improve hippocampal redox status in aged female rats.

Behavioural brain research

Wang J,Yu R,Han QQ,Huang HJ,Wang YL,Li HY,Wang HM,Chen XR,Ma SL,Yu J

Fri Feb 01 00:00:00 EST 2019

Triptolide interferes with XRCC1/PARP1-mediated DNA repair and confers sensitization of triple-negative breast cancer cells to cisplatin.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Zhang Z,Sun C,Zhang L,Chi X,Ji J,Gao X,Wang Y,Zhao Z,Liu L,Cao X,Yang Y,Mao W

Tue Jan 01 00:00:00 EST 2019

Anti-MET VHH Pool Overcomes MET-Targeted Cancer Therapeutic Resistance.

Molecular cancer therapeutics

Su Z,Han Y,Sun Q,Wang X,Xu T,Xie W,Huang X

Tue Jan 01 00:00:00 EST 2019

The synergistic effect of NOD2 and TLR4 on the activation of autophagy in human submandibular gland inflammation.

Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology

Li J,Li B,Cheng Y,Meng Q,Wei L,Li W,Zhang J,Huang S

Tue Jan 01 00:00:00 EST 2019

Identification of a cellularly active SIRT6 allosteric activator.

Nature chemical biology

Huang Z,Zhao J,Deng W,Chen Y,Shang J,Song K,Zhang L,Wang C,Lu S,Yang X,He B,Min J,Hu H,Tan M,Xu J,Zhang Q,Zhong J,Sun X,Mao Z,Lin H,Xiao M,Chin YE,Jiang H,Xu Y,Chen G,Zhang J

Sat Dec 01 00:00:00 EST 2018

Computational and biochemical studies of isothiocyanates as inhibitors of proteasomal cysteine deubiquitinases in human cancer cells.

Journal of cellular biochemistry

Ahmed ZSO,Li X,Li F,Cheaito HA,Patel K,Mosallam EM,Elbargeesy GAEH,Dou QP

Thu Nov 01 00:00:00 EDT 2018

Interfilament interaction between IMPDH and CTPS cytoophidia.

The FEBS journal

Chang CC,Keppeke GD,Sung LY,Liu JL

Mon Oct 01 00:00:00 EDT 2018

HMGB1-induced autophagy facilitates hepatic stellate cells activation: a new pathway in liver fibrosis.

Clinical science (London, England : 1979)

Li J,Zeng C,Zheng B,Liu C,Tang M,Jiang Y,Chang Y,Song W,Wang Y,Yang C

Thu Aug 16 00:00:00 EDT 2018

Adaptive mutation F772S-enhanced p7-NS4A cooperation facilitates the assembly and release of hepatitis C virus and is associated with lipid droplet enlargement.

Emerging microbes & infections

Duan X,Anwar MI,Xu Z,Ma L,Yuan G,Chen Y,Liu X,Xia J,Zhou Y,Li YP

Wed Aug 08 00:00:00 EDT 2018

PTEN Regulates Nonhomologous End Joining By Epigenetic Induction of NHEJ1/XLF.

Molecular cancer research : MCR

Sulkowski PL,Scanlon SE,Oeck S,Glazer PM

Wed Aug 01 00:00:00 EDT 2018

Loss of the ubiquitin conjugating enzyme UBE2E3 induces cellular senescence.

Redox biology

Plafker KS,Zyla K,Berry W,Plafker SM

Sun Jul 01 00:00:00 EDT 2018

Vitexin reduces epilepsy after hypoxic ischemia in the neonatal brain via inhibition of NKCC1.

Journal of neuroinflammation

Luo WD,Min JW,Huang WX,Wang X,Peng YY,Han S,Yin J,Liu WH,He XH,Peng BW

Wed Jun 20 00:00:00 EDT 2018

Identifying the SUMO1 modification of FAM122A leading to the degradation of PP2A-Cα by ubiquitin-proteasome system.

Biochemical and biophysical research communications

Fan F,Zhao J,Liu Y,Zhao H,Weng L,Li Q,Chen G,Xu Y

Thu Jun 07 00:00:00 EDT 2018

Expression of nerve growth factor carried by pseudotyped lentivirus improves neuron survival and cognitive functional recovery of post-ischemia in rats.

CNS neuroscience & therapeutics

Cao JY,Lin Y,Han YF,Ding SH,Fan YL,Pan YH,Zhao B,Guo QH,Sun WH,Wan JQ,Tong XP

Fri Jun 01 00:00:00 EDT 2018

Gelofusine Attenuates Tubulointerstitial Injury Induced by cRGD-Conjugated siRNA by Regulating the TLR3 Signaling Pathway.

Molecular therapy. Nucleic acids

Cen B,Liao W,Wang Z,Gao L,Wei Y,Huang W,He S,Wang W,Liu X,Pan X,Ji A

Fri Jun 01 00:00:00 EDT 2018

[6]-Gingerol enhances the radiosensitivity of gastric cancer via G2/M phase arrest and apoptosis induction.

Oncology reports

Luo Y,Chen X,Luo L,Zhang Q,Gao C,Zhuang X,Yuan S,Qiao T

Tue May 01 00:00:00 EDT 2018

Ubiquitin-specific protease 15 promotes tumor cell invasion and proliferation in glioblastoma.

Oncology letters

Xu K,Pei H,Zhang Z,Wang H,Li L,Xia Q

Thu Mar 01 00:00:00 EST 2018

Homeobox B3 promotes tumor cell proliferation and invasion in glioblastoma.

Oncology letters

Xu K,Qiu C,Pei H,Mehmood MA,Wang H,Li L,Xia Q

Thu Mar 01 00:00:00 EST 2018

Transcriptional regulation of P63 on the apoptosis of male germ cells and three stages of spermatogenesis in mice.

Cell death & disease

Wang H,Yuan Q,Niu M,Zhang W,Wen L,Fu H,Zhou F,He Z

Tue Jan 23 00:00:00 EST 2018

The Significant Role of c-Abl Kinase in Barrier Altering Agonists-mediated Cytoskeletal Biomechanics.

Scientific reports

Wang X,Wang L,Garcia JGN,Dudek SM,Shekhawat GS,Dravid VP

Wed Jan 17 00:00:00 EST 2018

Lipopolysaccharide (LPS)-mediated priming of toll-like receptor 4 enhances oxidant-induced prostaglandin E₂ biosynthesis in primary murine macrophages.

International immunopharmacology

Zhang Y,Igwe OJ

Mon Jan 01 00:00:00 EST 2018

Progranulin derived engineered protein Atsttrin suppresses TNF-α-mediated inflammation in intervertebral disc degenerative disease.

Oncotarget

Ding H,Wei J,Zhao Y,Liu Y,Liu L,Cheng L

Tue Dec 12 00:00:00 EST 2017

Twinfilin 1 enhances milk bio-synthesis and proliferation of bovine mammary epithelial cells via the mTOR signaling pathway.

Biochemical and biophysical research communications

Li L,Liu L,Qu B,Li X,Gao X,Zhang M

Sat Oct 21 00:00:00 EDT 2017

DNMT1-maintained hypermethylation of Krüppel-like factor 5 involves in the progression of clear cell renal cell carcinoma.

Cell death & disease

Fu RJ,He W,Wang XB,Li L,Zhao HB,Liu XY,Pang Z,Chen GQ,Huang L,Zhao KW

Thu Jul 27 00:00:00 EDT 2017

Critical roles of CTP synthase N-terminal in cytoophidium assembly.

Experimental cell research

Huang Y,Wang JJ,Ghosh S,Liu JL

Mon May 15 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; Atcb; B-ACTZF; beta cytoskeletal actin; Beta-actin; Beta-actin-1; Cap Z; capZ beta; cytoplasmic beta-actin; cytoskeleton actin; gamma actin; PS1TP5-binding protein 1

Gene Aliases: ACTB; actba; Actx; B-ACTZF; bact; bactin1; bactzf; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1; wu:fd18f05

UniProt ID: (Human) P60709, (Rat) P60711, (Mouse) P60710, (Zebrafish) Q7ZVI7

Entrez Gene ID: (Human) 60, (Rat) 81822, (Mouse) 11461, (Zebrafish) 57934

Molecular Function: actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein