OriGene

beta Actin Monoclonal Antibody (OTI1), TrueMAB™

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beta Actin Antibody in Western Blot (WB) — Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-betbeta-actin monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). (1:2000)

Product Details

TA811000

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

Miscellaneous PubMed (Misc)

View 20 publications 20 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG3

Class

Monoclonal

Type

Antibody

Clone

OTI1

Immunogen

Synthetic peptide corresponding to the N terminal of human beta-actin conjugated to KLH.

View immunogen

Conjugate

Unconjugated

Form

liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Target Information

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; Atcb; beta cytoskeletal actin; Beta-actin; Cap Z; capZ beta; cytoplasmic beta-actin; cytoskeleton actin; gamma actin; PS1TP5-binding protein 1

Gene Aliases: ACTB; Actx; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1

UniProt ID: (Human) P60709, (Dog) O18840, (Rat) P60711, (Mouse) P60710

Entrez Gene ID: (Human) 60, (Dog) 403580, (Rat) 81822, (Mouse) 11461

Molecular Function: actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein