Immunofluorescent analysis of beta Amyloid showing staining in the cytoplasm of A431 cells. A431 cells were fixed in ice-cold MeOH for 5 min and stained using a beta Amyloid polyclonal antibody (Product # PA5-34657) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 602 and 770 of beta Amyloid (Uniprot ID#P05067)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34657 targets beta Amyloid in ICC, IF, IHC (P), and WB applications and shows reactivity with Human and Mouse samples.
The PA5-34657 immunogen is recombinant fragment corresponding to a region within amino acids 602 and 770 of beta Amyloid (Uniprot ID#P05067).
The protein encoded by this gene interacts with the cytoplasmic domains of amyloid beta (A4) precursor protein and amyloid beta (A4) precursor-like protein 2. This protein contains two phosphotyrosine binding (PTB) domains, which are thought to function in signal transduction. Polymorphisms in this gene have been associated with Alzheimer's disease. Alternative splicing results in multiple transcript variants.
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