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Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500 | - |
Immunocytochemistry (ICC/IF) |
2 µg/mL | - |
ELISA (ELISA) |
Assay-dependent | - |
Miscellaneous PubMed (Misc) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Mouse |
Host/Isotype |
Mouse / IgG1, kappa |
Class |
Monoclonal |
Type |
Antibody |
Clone |
ZA005 |
Immunogen |
Synthetic peptide derived from the C-terminal region of human beta-arrestin 1 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS, pH 7.4 |
Contains |
0.1% sodium azide |
Storage conditions |
-20°C |
RRID |
AB_2533416 |
Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold molecule, beta Arrestin 1 is essential for mitogenic signaling and mediates agonist-dependent desensitization and internalization of Gprotein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosphorylated by G-protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1 and 2 on serine412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1 and 2 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: arrestin; arrestin 2; Arrestin beta-1; arrestin, beta 1; beta-arrestin; Beta-arrestin-1; beta-arrestin1; Non-visual arrestin-2
Gene Aliases: 1200006I17Rik; ARB1; ARR1; ARRB1; AW208571; BARRES; G430100A01Rik
UniProt ID: (Human) P49407, (Rat) P29066, (Mouse) Q8BWG8
Entrez Gene ID: (Human) 408, (Rat) 25387, (Mouse) 109689
Molecular Function:
scaffold/adaptor protein
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