|Tested species reactivity||Hamster, Human, Non-human primate, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to amino acids 701-715 of beta COP.|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Western Blot (WB)||1/1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA1-22151 detects Beta-COP in hamster, human, rat and monkey samples.
MA1-22151 has been successfully used in immunocytochemistry, immunofluorescence, immunohistochemistry (formalin and paraffin), and Western blot procedures. In immunofluorescence, use methanol/acetone or paraformaldehyde fixation, followed by a permeabilization step. Strong immunofluorescent staining is confined mainly to the central perinuclear area in the cell.
MA1-22151 immunogen corresponds to a synthetic peptide, conjugated to KLH, corresponding to amino acids 701-715 of beta COP.
This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Golgi fragmentation is Rab and SNARE dependent in cellular models of Parkinson's disease.
MA1-22151 was used in western blot to study the role of Rab and SNARE proteins in the Golgi fragmentation observed in models of Parkinson's disease
|Rendón WO,Martínez-Alonso E,Tomás M,Martínez-Martínez N,Martínez-Menárguez JA||Histochemistry and cell biology (139:671)||2013|