Immunofluorescent analysis of Beta-Catenin using Beta-Catenin Monoclonal antibody (9F2) (Product# MA1-2001) shows staining in U251 glioma cells. Beta-Catenin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Beta-Catenin (Product# MA1-2001) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||120 kDa MBP/beta catenin fusion protein purified on amylose column.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||10 µg/ml|
|Immunofluorescence (IF)||10 ug/ml|
|Immunoprecipitation (IP)||1 ug/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
MA1-2001 detects beta catenin from human samples.
MA1-2001 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~94 kDa protein representing beta catenin from A431 cell extract. MA1-2001 can also be used in immunoprecipitation and immunofluorescence procedures.
The MA1-2001 immunogen is a 120 kDa MBP/ beta catenin fusion protein purified via an amylose column.
The Catenins (alpha, beta and gamma) are ubiquitously expressed cytoplasmic proteins, which are associated with E-cadherin. Beta Cadherin can also bind to N-cadherin and co-immunoprecipitates with APC. Cadherin/Catenin complexes are linked to the cytoskeleton via a direct association between alpha Actinin and alpha Catenin.
IP-MS enrichment of CTNNB1 (LFQ intensity): CTNNB1 was enriched 18-fold from HCT116 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and CTNNB1 antibody (Product # MA1-2001). STRING database was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin.
MA1-2001 was used in immunocytochemistry and western blot to investigate the interaction of plakoglobin with N-cadherin and alpha catenin
|Sacco PA,McGranahan TM,Wheelock MJ,Johnson KR||The Journal of biological chemistry (270:20201)||1995|
|Not Applicable||Not Cited||
Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.
MA1-2001 was used in immunoprecipitation and western blot to investigate the interaction between alpha-actinin and N-cadherin/catenin complex
|Knudsen KA,Soler AP,Johnson KR,Wheelock MJ||The Journal of cell biology (130:67)||1995|