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HeLa cells were plated on coverslips overnight. The next day the cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol. Cells were then incubated with 3 µg/mL anti-B-catenin antibody (712700) for 60 min at room temperature followed by three washes with dPBS. Cells were then incubated with a 1:1000 dilution of goat anti-rabbit Qdot® 655 secondary antibody (Q11421MP) for 60 min, followed by three washes with dPBS. Cells were labeled with NucBlue® Live cell stain (R37605) and ActinGreen™ 488 ReadyProbes® reagent (R37110) according to protocol. Coverslips were then mounted using ProLong® Gold Antifade Reagent (P36930). Images were taken using the EVOS® FL Auto Imaging System and a 40X Coverslip corrected objective (AMEP4699).
|Tested species reactivity||Mouse , Rat , Human , Chicken , Clawed frog|
|Published species reactivity||Rat , Mouse , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 15 amino acid synthetic peptide derived from the C-terminus of the human/ mouse beta-catenin protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Liver-Tumor Hybrid Organoids for Modeling Tumor Growth and Drug Response In Vitro.
71-2700 was used in immunocytochemistry and immunohistochemistry to investigate using liver-tumor organoids for in vitro modeling of liver metastasis
|Skardal A,Devarasetty M,Rodman C,Atala A,Soker S||Annals of biomedical engineering (43:2361)||2015|
Actin-bundling protein plastin 3 is a regulator of ectoplasmic specialization dynamics during spermatogenesis in the rat testis.
71-2700 was used in immunohistochemistry and western blot to study the role of plastin 3 in ectoplasmic specialization dynamics during spermatogenesis in the rat testis
|Li N,Mruk DD,Wong CK,Lee WM,Han D,Cheng CY||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (29:3788)||2015|
Musashi-1 maintains blood-testis barrier structure during spermatogenesis and regulates stress granule formation upon heat stress.
71-2700 was used in western blot to study the role of Musashi-1 in blood-testis barrier structure during spermatogenesis
|ErLin S,WenJie W,LiNing W,BingXin L,MingDe L,Yan S,RuiFa H||Molecular biology of the cell (26:1947)||2015|
rpS6 regulates blood-testis barrier dynamics through Akt-mediated effects on MMP-9.
||Mok KW,Mruk DD,Cheng CY||Journal of cell science (127:4870)||2014|
Regulation of the blood-testis barrier by coxsackievirus and adenovirus receptor.
||Su L,Mruk DD,Cheng CY||American journal of physiology. Cell physiology (303:C843)||2012|
Flutamide alters the distribution of c-Src and affects the N-cadherin-β-catenin complex in the seminiferous epithelium of adult rat.
71-2700 was used in immunohistochemistry - paraffin section, immunoprecipitation, and western blot to study the effect of flutamide on the distribution of c-Src and the N-cadherin-beta-catenin complex in the seminiferous epithelium of adult rat
|Zarzycka M,Chojnacka K,Mruk DD,Gorowska E,Hejmej A,Kotula-Balak M,Pardyak L,Bilinska B||Andrology (3:569)||2015|
Cadmium alters the localization of N-cadherin, E-cadherin, and beta-catenin in the proximal tubule epithelium.
71-2700 was used in immunohistochemistry - frozen section to test if Cd affects cadherin-dependent junctions in the proximal tubule epithelium in vivo.
|Prozialeck WC,Lamar PC,Lynch SM||Toxicology and applied pharmacology (189:180)||2003|
Is the cadherin/catenin complex a functional unit of cell-cell actin-based adherens junctions in the rat testis?
71-2700 was used in immunohistochemistry - frozen section to show that the cadherin/catenin complex is present between Sertoli and germ cells and is used for the assembly of functional adheren junctions.
|Lee NP,Mruk D,Lee WM,Cheng CY||Biology of reproduction (68:489)||2003|
CHBCAT, Bfc, armadillo, ctnnb1, beta-catenin, ctnnb, CTNNB, Mesc, OK/SW-cl.35, Catnb, MRD19
beta-catenin, catenin beta-1, catenin (cadherin associated protein), beta 1, 88kDa, catenin beta, beta catenin, CATNB, CTNB1, CTNNB, catenin, beta