|Tested species reactivity||Chicken, Human, Mouse, Rat, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from the C-terminus of the beta-catenin protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Immunoprecipitation (IP)||10 µg/ml|
|Western Blot (WB)||5-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16429 targets Catenin-beta in immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with mouse, rat, Xenopus, chicken, and human samples.
The PA5-16429 immunogen is a synthetic peptide from the C-terminus of the beta-catenin protein.
The catenins (alpha, beta and gamma) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions. beta-catenin also binds to N-cadherin and co-immunoprecipitates with APC. Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between alpha-actinin and alpha-catenin. Increased tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion.
IP-MS enrichment of CTNNB1 (LFQ intensity): CTNNB1 was enriched 68-fold from HCT116 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and CTNNB1 antibody (Product # PA5-16429). STRING database was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.