Immunohistochemical (formalin-fixed, paraffin-embedded) staining of Human Liver tissue using PA5-19469, anti-Beta Catenin antibody. Primary antibody was used at a concentration of 1ug/ml and exposed for 15 mins at room temp. The sample was pretreated using heat mediated antigen retrieval with Sodium Citrate Buffer (pH6 / 20mins). The detection method was a HRP conjugated polymer, DAB chromogen and the sample was counterstained with haematoxylin and mounted with DPX.
|Tested species reactivity||Human, Mouse, Rodent, Rat, Xenopus, Zebrafish|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human beta Catenin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1 µg/ml|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1 µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||0.25 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with chicken and pig based on sequence homology.
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions . AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer , pilomatrixoma , medulloblastoma , and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.
IP-MS enrichment of CTNNB1 (LFQ intensity): CTNNB1 was enriched 345-fold from HCT116 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and CTNNB1 antibody (Product # PA5-19469). STRING database was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Vesicular Galectin-3 levels decrease with donor age and contribute to the reduced osteo-inductive potential of human plasma derived extracellular vesicles.
PA5-19469 was used in western blot to determine reduction of osteo-inductive potential of human plasma derived extracellular vesicles by a decrease in vesicular galectin-3 levels that decreses with donor age
|Weilner S,Keider V,Winter M,Harreither E,Salzer B,Weiss F,Schraml E,Messner P,Pietschmann P,Hildner F,Gabriel C,Redl H,Grillari-Voglauer R,Grillari J||Aging (8:16)||2016|