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Immunofluorescent analysis of Catenin beta in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Catenin beta monoclonal antibody (Product # MA1-301) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Catenin beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
|Tested species reactivity||Human , Rat , Mouse , Primate|
|Host / Isotype||Mouse|
|Immunogen||Recombinant beta Catenin protein.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Gel Shift (GS)||Assay Dependent|
|Immunofluorescence (IF)||1:50 - 1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100 - 1:1000|
|Western Blot (WB)||1:500 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-301 detects beta Catenin from human, mouse, monkey, and rat samples.
MA1-301 has been successfully used in Western blot, gel shift, immunohistochemistry (paraffin), and immunofluorescence applications. By Western blot this antibody detects a ~94 kDa protein representing beta Catenin.
Beta Catenin is thought to play a role in cell-cell communication and adhesion, and possibly cell morphology. In the nucleus, beta Catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. This influence on cell proliferation is the molecular basis for the role of beta Catenin in tumorgenesis, in particular solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bfc, armadillo, CTNNB, Mesc, OK/SW-cl.35, Catnb, MRD19
catenin beta-1, beta-catenin, catenin (cadherin associated protein), beta 1, 88kDa, catenin beta, Cadherin associated protein, Catenin beta 1, CATNB, CTNNB, beta-catenin, beta catenin, CTNNB1