|ELISA (ELISA)||0.1-1 mg/mL|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Immunoprecipitation (IP)||5-10 µg|
|Western Blot (WB)||1-2 µg/mL|
|Immunocytochemistry (ICC)||See 8 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
|Immunofluorescence (IF)||See 6 publications below|
|Western Blot (WB)||See 14 publications below|
|Immunoprecipitation (IP)||See 3 publications below|
|Miscellaneous PubMed (MISC)||See 5 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|ChIP assay (ChIP)||See 1 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 5 publications below|
|Species reactivity||Chicken, Human, Mouse, Rat, Xenopus|
|Published species||Dog, Human, Mouse, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 15-amino acid synthetic peptide derived from the C-terminus of the human/mouse beta-catenin protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
This antibody can be used to specifically immunoprecipitate the ~ 92 kDa beta-catenin protein from native cell lysates. It is suitable for use in immunoprecipitation (IP) and IP/western applications. Note that when the antibody is used for straight western blotting, cross-reactivity with a ~100 kDa protein of unknown identity is sometimes observed. Suggested positive control lysates include HeLa and A431.
The protein, beta-catenin, encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs) (also referred to as zonula adherens). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. In addition, these junctions have been identified in several other cell types including cardiac myocytes and fibroblasts. AJ subserve several important functions including: mediating intercellular adhesion and sensing the presence of neighboring cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. AJs are multiprotein complexes that are assembled around cell adhesion molecules called cadherins. Cadherins are a multifunctional family of Ca2+-dependent transmembrane glycoproteins which promote cell-cell adhesion. The cadherin extracellular domain mediates homophilic interactions between like cadherin molecules on neighboring cells, while the intracellular domain interacts with several cytoplasmic proteins which include: alpha-catenin, beta-catenin, gamma-catenin (plakoglobin), and the tyrosine kinase substrate p120cas. Cadherin-catenin interactions are required for complete cadherin activity and regulate the interaction between cadherins and the actin-based cytoskeleton. In fact, deletion of the cadherin cytoplasmic domain produces an adhesion-defective molecule that is unable to interact with the cytoskeleton. Beta-catenin is a 92 kDa protein which shares 70% amino acid identity with both plakoglobin and the product of the Drosophila segment polarity gene armadillo. The Armadillo protein is part of a multiprotein junctional complex and is a required component of the Drosophilia wingless (vertebrate Wnt-1) signal transduction pathway. Beta-catenin, gamma-catenin, and p120cas are homologous but distinct proteins which contain between 10-13 copies of a 42-44 amino acid motif first identified in the Armadillo protein and referred to as armadillo repeats. These armadillo repeats mediate the interaction between beta- and gamma-catenin and the cadherin cytoplasmic domain. Finally, beta-catenin binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene. Interestingly, the interaction between APC and beta-catenin has been speculated to ""regulate the transmission of the contact inhibition signal to the cell"". Taken together these findings suggest that beta-catenin likely plays a central role in signal transduction pathways regulated by cell-cell adhesion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: beta; beta catenin; Beta-catenin; catenin; catenin (cadherin associated protein), beta 1, 88kDa; catenin (cadherin-associated protein), beta 1; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta; Catenin beta-1; CATNB; CTNB1; CTNNB
Gene Aliases: armadillo; beta-catenin; Bfc; Catnb; CHBCAT; CTNNB; CTNNB1; Mesc; MRD19; OK/SW-cl.35; PRO2286
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