Immunoprecipitation of beta CstF-64 variant in HeLa lysate, either in RIPA buffer (lanes 1, 2, 4, 6) or TX100 buffer (lanes 3, 5, 7). Lysates overexpressing mouse beta-Cstf-64 (DYKDDDDK tagged) immunoprecipitated using 2ug of b-CstF-64 polyclonal antibody (Product# PA5-37985) with Protein G-coated magnetic beads (lanes 6,7). Samples were probed with a DYKDDDDK antibody at a dilution of 1:1000. Lane 1 shows mock-transfected HeLa cells. A 1/40th section of the lysate was loaded onto lanes 2 and 3 before IP, and in lanes 4 an 5 following IP.
|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-PASIERVQGQRT, from the internal region of mouse Cstf2|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2µg/500µg lysate|
|Western Blot (WB)||2µg|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.
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