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beta Galactosidase Antibody (200-901-036) in WB
Western Blot results of Chicken Anti-Beta Galactosidase Antibody. Lane 1: Opal Prestained Molecular Weight Ladder (p/n MB-210-0500). Lane 2: Beta Galactosidase. Load: 50ng. Primary Antibody: Rabbit Anti-Beta Galactosidase at 1µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Chicken HRP (p/n 603-103-126) at 1:40,000 for 30min at RT. Blocking: BlockOut (p/n MB-073) for 30 min at RT.
Product Details
200-901-036
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Antibody
Immunogen
This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with full length native Beta Galactosidase isolated from E.coli
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
selective precipitation
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This Anti-Beta Galactosidase Antibody is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Beta Galactosidase [E.coli] and anti-Chicken Serum. Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.
Target Information
Beta Galactosidase encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene have been associated with GM1-gangliosidosis and Morquio B syndrome. Diseases associated with GLB1 include Gm1-Gangliosidosis, Type I and Gm1-Gangliosidosis, Type Ii.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Beta-Gal; betagal; bgal; ß-gal; ß-galactosidase; ßgal
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