|Tested species reactivity||Eubacteria|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant full length beta Galactosidase protein expressed in E. coli.|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Dot blot (DB)||Assay dependent|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||1:500-1:3000|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:2000-1:5000|
PA1-21477 detects beta Galactosidase in E. coli samples.
PA1-21477 has been successfully used in ELISA, immunocytochemistry, immunofluorescence, immunohistochemistry (frozen and FFPE), immunoprecipitation, dot blot and Western blot procedures.
The PA1-21477 immunogen corresponds to recombinant full length beta Galactosidase protein expressed in E. coli.
Store at 4°C short term. For extended storage, store at -20°C, avoiding freeze/thaw cycles.
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
Beta-Gal; GLB2; GSL; NGBE; PPCA; PPGB; RP3-337O18.1