|Tested species reactivity||Bacteria|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant full length beta Galactosidase protein expressed in E. coli.|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Dot blot (DB)||Assay dependent|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:500-1:3000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500-1:3000|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:2000-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 1 publications below|
PA1-21477 detects beta Galactosidase in E. coli samples.
PA1-21477 has been successfully used in ELISA, immunocytochemistry, immunofluorescence, immunohistochemistry (frozen and FFPE), immunoprecipitation, dot blot and Western blot procedures.
The PA1-21477 immunogen corresponds to recombinant full length beta Galactosidase protein expressed in E. coli.
Store at 4°C short term. For extended storage, store at -20°C, avoiding freeze/thaw cycles.
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A novel one-step expression and immobilization method for the production of biocatalytic preparations.
PA1-21477 was used in ELISA to discuss biocatalytic preparations for industrial applications
|Sührer I,Langemann T,Lubitz W,Weuster-Botz D,Castiglione K||Microbial cell factories (14:null)||2015|