Immunofluorescent analysis of beta IV Tubulin in paraformaldehyde-fixed U2OS cells using a beta IV Tubulin polyclonal antibody (Product # PA5-21416) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment contain a sequence corresponding to a region within amino acids 238 and 410 (P04350) of beta Tubulin 4|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-21416 targets beta IV Tubulin in ICC, IF, and WB applications and shows reactivity with Human and mouse samples.
The PA5-21416 immunogen is recombinant protein fragment contain a sequence corresponding to a region within amino acids 238 and 410 (P04350) of beta Tubulin 4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.