|Tested species reactivity||Bacteria|
|Host / Isotype||Mouse / IgG1|
|Immunogen||5'-His-tagged E.coli TEM-1 beta lactamase.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||10-20 µg/ml|
|Western Blot (WB)||5-15 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-20370 detects beta lactamase in bacteria samples.
MA1-20370 has been successfully used in ELISA and Western blot procedures.
The MA1-20370 immunogen is 5'-His-tagged E.coli TEM-1 beta lactamase.
The beta lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents. All of the beta lactams are structurally related through the presence of a core beta lactam ring. Bacterial resistance to beta lactams continues to increase, primarily due to the production of beta lactamases. Beta lactamases catalyze the hydrolysis of the beta lactam bond, which destroys antibacterial activity. Bacteria that produce TEM type or SHV type beta lactamases have point mutations in structural genes that have extended the substrate specificity of these beta lactamases. As a result, many of the beta lactamase producing Gram negative pathogens have become multidrug resistant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.