Invitrogen

beta Tubulin 2,4 Recombinant Rabbit Monoclonal Antibody (ST52-04)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Protocols

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beta Tubulin 2,4 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Tubulin beta-2A chain was performed using 70% confluent log phase NTERA-2 cl.D1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with beta Tubulin 2,4 Recombinant Rabbit Monoclonal Antibody (ST52-04) (Product # MA5-32163) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoskeletal localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

beta Tubulin 2,4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of beta Tubulin 2,4 of paraffin-embedded Human kidney tissue using a beta-Tubulin-2-4 Monoclonal antibody (Product #MA5-32163). Counter stained with hematoxylin.

beta Tubulin 2,4 Antibody in Western Blot (WB) — Western Blot was performed using Anti-beta Tubulin 2,4 Recombinant Rabbit Monoclonal Antibody (ST52-04) (Product # MA5-32163) and a 50 kDa band corresponding to Tubulin beta-2A chain was observed across in NTERA2, Mouse Brain and Rat Brain, but not in the other cell line and tissue models. Whole cell extracts (30 µg lysate) of NTERA-2 cl.D1 (Lane 1), Caco-2 (Lane 2), BJ (Lane 3), HeLa (Lane 4), HL-60 (Lane 5), THP-1 (Lane 6), Mouse Brain (Lane 7), Mouse Ovary (Lane 8), Rat Brain (Lane 9), Rat Ovary (Lane 10) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:3000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

beta Tubulin 2,4 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across the tested cell lines and tissues owing to their inherent genetic constitution. Relative expression of Tubulin beta-2A chain was observed in Mouse Brain and Rat Brain, but not in Mouse Ovary and Rat Ovary using Anti-beta Tubulin 2,4 Recombinant Rabbit Monoclonal Antibody (ST52-04) (Product # MA5-32163) in Western Blot. {RE}

Product Details

MA5-32163

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:5,000	-
Immunohistochemistry (IHC)	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Expression System	HEK293 cells
Class	Recombinant Monoclonal
Type	Antibody
Clone	ST52-04
Immunogen	Synthetic peptide within Human beta II Tubulin aa 407-445 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2809452

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: beta-tubulin T beta15 (aa 1-445); class II beta-tubulin isotype; class IIa beta-tubulin; class IIb beta-tubulin; class IVb beta tubulin; class IVb beta-tubulin; dystonia 4, torsion (autosomal dominant); novel tubulin protein; T beta-15; tubb2a {ECO:0000250|UniProtKB:Q7TMM9}; Tubulin 5 beta; tubulin beta; Tubulin beta class IIa; Tubulin beta-2 chain; Tubulin beta-2A chain; Tubulin beta-2B chain; Tubulin beta-2C chain; Tubulin beta-4 chain; Tubulin beta-4A chain; Tubulin beta-4B chain; tubulin, beta 2; tubulin, beta 2A class IIa; tubulin, beta 2B class IIb; tubulin, beta 2C; tubulin, beta 2c1; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta 4A class IVa; tubulin, beta 4B class IVb; tubulin, beta polypeptide; tubulin, beta polypeptide 2; tubulin, beta polypeptide paralog; tubulin, beta, 2; tubulin, beta, 5

Gene Aliases: 2410129E14Rik; 4930542G03Rik; AI325297; bA506K6.1; beta-5; Beta2; brdp; CDCBM5; DYT4; M(beta)2; M(beta)4; PMGYSA; RGD1309427; TUBB; TUBB2; TUBB2A; TUBB2B; TUBB2C; Tubb2c1; TUBB4; TUBB4A; TUBB4B; TUBB5

UniProt ID: (Human) Q13885, (Human) Q9BVA1, (Human) P04350, (Human) P68371, (Rat) P85108, (Mouse) Q7TMM9, (Mouse) Q9CWF2, (Rat) Q3KRE8, (Mouse) Q9D6F9, (Rat) Q6P9T8, (Mouse) P68372