|Immunocytochemistry (ICC)||1-3 µg/ml|
|Immunofluorescence (IF)||1-3 µg/mL|
|Western Blot (WB)||1-3 µg/mL|
|Immunocytochemistry (ICC)||See 5 publications below|
|Western Blot (WB)||See 29 publications below|
|Miscellaneous PubMed (MISC)||See 5 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Dog, C. elegans, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Dog , Rat , Mouse , Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Clone||2 28 33|
|Immunogen||Beta-tubulin from sea urchin (S. purpuratus) sperm.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
This antibody reacts with the ~50 kDa beta-tubulin and has been shown to bind to the two major and one of the minor beta-tubulin isotypes. Reactivity has been confirmed with mouse NIH3T3 fibroblast cells, rat brain, and mouse testis.
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules. The tubulin protein is a major target of drug molecules, and consequently, tubulin inhibitors have attracted great attention as antimitotic antitumor agents for chemotherapeutic use. The effects on tubulin messenger RNA levels and tubulin protein synthesis when treating cells with microtubule-depolymerizing drugs or when directly microinjecting cells with tubulin suggest that non-polymerized tubulin depresses its own synthesis. Accumulation of tubulin protein and an increased array of microtubules have been associated with contractile dysfunction in cardiac myocytes after pressure overload in vivo. Studies have also shown that cardiac activity can increase the amount of beta tubulin in rat cardiac myocytes. Tubulin production in cultured cardiac myocytes can be regulated directly by mechanical forces. In mechanically challenged hearts, the accumulation of beta tubulin and the development of contractile dysfunction may be directly related to the mechanical forces imposed on the myocardium during the onset and progression of cardiovascular disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: beta 3; beta 3 class III; beta Ib tubulin; beta-4; CDCBM; CFEOM3A; class I beta-tubulin; class III beta-tubulin; TUBB4; tubulin; Tubulin beta chain; Tubulin beta-1 chain; tubulin beta-3 chain; Tubulin beta-5 chain; tubulin, beta 1 class VI; tubulin, beta 2A class IIa; tubulin, beta class I; tubulin, beta polypeptide
Gene Aliases: 2810484G07Rik; CDCBM6; CSCSC1; M(beta)1; M40; OK/SW-cl.56; TUBB; TUBB1; TUBB5
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