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Immunofluorescence analysis of neurons using anti-beta III tubulin antibody (480011). GABAergic precursor cells derived from H9 embryonic stem cells were differentiated for 7 days and stained with antibodies against GABA (Cat. No. PA5-32241 at 1:5000) followed by Alexa Fluor® 488 goat anti-rabbit (Cat. No. A21206, green) and neuronal marker beta III tubulin (Cat. No. 480011 at 1:1000) followed by by Alexa Fluor® 594 goat-anti-mouse (Cat. No. A11005, red). Nuclear DNA was stained with DAPI (blue).
|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG|
|Immunogen||Synthetic peptide corresponding to beta III tubulin|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||1:10000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
FHL1 reduces dystrophy in transgenic mice overexpressing FSHD muscular dystrophy region gene 1 (FRG1).
480011 was used in western blot to assess how FRG1 expression contributes to myoblast differentiation defects
|Feeney SJ,McGrath MJ,Sriratana A,Gehrig SM,Lynch GS,D'Arcy CE,Price JT,McLean CA,Tupler R,Mitchell CA||PloS one (10:null)||2015|
beta 3; beta 3 class III; beta Ib tubulin; beta-4; CDCBM; CFEOM3A; class III beta-tubulin; TUBB4; tubulin; tubulin beta-1 chain; tubulin beta-3 chain; tubulin beta-5 chain; tubulin, beta class I; tubulin, beta polypeptide
CDCBM6; CSCSC1; M40; OK/SW-cl.56; TUBB; TUBB1; TUBB5