Immunofluorescent analysis of beta-tubulin (green) in HeLa cells. Cells were fixed and permeabilized with ice-cold methanol for 10 minutes at room temperature, and blocked with 0.3% BSA in PBS for at least 15 minutes at room temperature. Cells were probed with a beta-tubulin monoclonal antibody (Product # MA5-11732) at a dilution of 1:100 (right panel), or incubated in blocking buffer as a negative control (left panel) overnight at 4°C. Cells were washed with PBS, and incubated with a DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for at least 1 hour at room temperature. Nuclei (blue) were stained with DAPI (Product # 46190). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Amphibian, Bovine, Chicken, Human, Insect, Mouse, Non-human primate, Plant, Rabbit, Rat|
|Published species reactivity||Plant, Mouse, Human|
|Host / Isotype||Mouse / IgG3|
|Clone||TBN06 (Tub 2.5)|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Western Blot (WB)||0.5-1.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11732 targets Tubulin Beta in IF, IHC (P), and WB applications and shows reactivity with Amphibian, Bovine, Chicken, Human, Insect, mouse, Plant, Rabbit, and Rat samples.
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Live imaging of prions reveals nascent PrPSc in cell-surface, raft-associated amyloid strings and webs.
MA5-11732 was used in immunocytochemistry to study the existence of PrPSC in cell surface strings and webs associated with lipid rafts using live imaging methodologies
|Rouvinski A,Karniely S,Kounin M,Moussa S,Goldberg MD,Warburg G,Lyakhovetsky R,Papy-Garcia D,Kutzsche J,Korth C,Carlson GA,Godsave SF,Peters PJ,Luhr K,Kristensson K,Taraboulos A||The Journal of cell biology (204:423)||2014|
A novel human dynactin-associated protein, dynAP, promotes activation of Akt, and ergosterol-related compounds induce dynAP-dependent apoptosis of human cancer cells.
MA5-11732 was used in immunocytochemistry to study the role dynAP, a dynactin-associated protein, in the induction of human cancer cell apoptosis by ergosterol-related compounds
|Kunoh T,Noda T,Koseki K,Sekigawa M,Takagi M,Shin-ya K,Goshima N,Iemura S,Natsume T,Wada S,Mukai Y,Ohta S,Sasaki R,Mizukami T||Molecular cancer therapeutics (9:2934)||2010|
Cytosolic carboxypeptidase 1 is involved in processing ¿- and ß-tubulin.
MA5-11732 was used in western blot to study the role of cytosolic carboxypeptidase 1 in processing Glu residues in ?- and ?-tubulin
|Berezniuk I,Vu HT,Lyons PJ,Sironi JJ,Xiao H,Burd B,Setou M,Angeletti RH,Ikegami K,Fricker LD||The Journal of biological chemistry (287:6503)||2012|
5-Aminoimidazole-4-carboxyamide ribonucleoside induces G(1)/S arrest and Nanog downregulation via p53 and enhances erythroid differentiation.
MA5-11732 was used in western blot to study the role of p53 in the mechanism by which the AMPK activator AICAR induces cell cycle arrest and promotes erythroid differentiation in murine embryonic stem cells
|Chae HD,Lee MR,Broxmeyer HE||Stem cells (Dayton, Ohio) (30:140)||2012|
Mechanism-based screen for G1/S checkpoint activators identifies a selective activator of EIF2AK3/PERK signalling.
MA5-11732 was used in western blot to identify and characterize small molecule inhibitors of G1/S checkpoint activation
|Stockwell SR,Platt G,Barrie SE,Zoumpoulidou G,Te Poele RH,Aherne GW,Wilson SC,Sheldrake P,McDonald E,Venet M,Soudy C,Elustondo F,Rigoreau L,Blagg J,Workman P,Garrett MD,Mittnacht S||PloS one (7:null)||2012|
Human subtilase SKI-1/S1P is a master regulator of the HCV Lifecycle and a potential host cell target for developing indirect-acting antiviral agents.
MA5-11732 was used in western blot to study the role of human subtilase SKI-1/S1P in the hepatitis C virus lifecycle
|Olmstead AD,Knecht W,Lazarov I,Dixit SB,Jean F||PLoS pathogens (8:null)||2012|
Trastuzumab induced in vivo tissue remodelling associated in vitro with inhibition of the active forms of AKT and PTEN and RhoB induction in an ovarian carcinoma model.
MA5-11732 was used in western blot to study the biological, molecular and cellular effects of Herceptin in ovarian carcinoma
|Delord JP,Quideau S,Rochaix P,Caselles O,Couderc B,Hennebelle I,Courbon F,Canal P,Allal BC||British journal of cancer (103:61)||2010|
HIV-1 Vpr induces TLR4/MyD88-mediated IL-6 production and reactivates viral production from latency.
MA5-11732 was used in western blot to investigate the effect of HIV-1 Vpr on interleukin 6 production and viral production
|Hoshino S,Konishi M,Mori M,Shimura M,Nishitani C,Kuroki Y,Koyanagi Y,Kano S,Itabe H,Ishizaka Y||Journal of leukocyte biology (87:1133)||2010|
L-type calcium channel blockers reverse docetaxel and vincristine-induced multidrug resistance independent of ABCB1 expression in human lung cancer cell lines.
MA5-11732 was used in western blot to investigate the relationship between ABCB1 expression and multidrug resistance induced by docetaxel or vincristine in human lung cancer cell lines
|Chiu LY,Ko JL,Lee YJ,Yang TY,Tee YT,Sheu GT||Toxicology letters (192:408)||2010|
KIT overexpression induces proliferation in astrocytes in an imatinib-responsive manner and associates with proliferation index in gliomas.
MA5-11732 was used in western blot to investigate the mechanism for the induction of astrocyte proliferation by KIT
|Blom T,Fox H,Angers-Loustau A,Peltonen K,Kerosuo L,Wartiovaara K,Linja M,Jänne OA,Kovanen P,Haapasalo H,Nupponen NN||International journal of cancer (123:793)||2008|
Modulation of TNF-alpha-converting enzyme by the spike protein of SARS-CoV and ACE2 induces TNF-alpha production and facilitates viral entry.
MA5-11732 was used in western blot to the role of SARS-CoV spike protein in the downregulation of angiotensin-converting enzyme 2 and its mechanism
|Haga S,Yamamoto N,Nakai-Murakami C,Osawa Y,Tokunaga K,Sata T,Yamamoto N,Sasazuki T,Ishizaka Y||Proceedings of the National Academy of Sciences of the United States of America (105:7809)||2008|
Dynamic regulation of endothelial NOS mediated by competitive interaction with alpha-actinin-4 and calmodulin.
MA5-11732 was used in western blot to investigate the mechanism of eNOS activity regulation in vascular endothelial cells
|Hiroi Y,Guo Z,Li Y,Beggs AH,Liao JK||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (22:1450)||2008|
Progressive changes in hepatoma cells stably transfected with hepatitis B virus X gene.
MA5-11732 was used in western blot to study the molecular mechanism of hepatocellular carcinoma development induced by hepatitis B virus X protein
|Ye L,Dong N,Wang Q,Xu Z,Cai N,Wang H,Zhang X||Intervirology (51:50)||2008|
S100A7 (Psoriasin), highly expressed in ductal carcinoma in situ (DCIS), is regulated by IFN-gamma in mammary epithelial cells.
MA5-11732 was used in western blot to study the signal transduction pathways involved in the regulation of psoriasin in murine mammary epithelial cells
|Petersson S,Bylander A,Yhr M,Enerbäck C||BMC cancer (7:null)||2007|
High expression of integrin beta1 correlates with high proliferation capacity in oral keratinocytes.
MA5-11732 was used in western blot to isolate two fractions of oral keratinocytes based on their affinity to collagen type IV to obtain cells with high prolifrative potential
|Stein E,Blaimauer K,Bauer S,Erovic BM,Turhani D,Thurnher D||Wiener klinische Wochenschrift (119:318)||2007|
Characterization of t(6;11)(p21;q12) in a renal-cell carcinoma of an adult patient.
MA5-11732 was used in western blot to report a clinical case of t(6;11)(p21;q12) renal-cell carcinoma
|Pecciarini L,Cangi MG,Lo Cunsolo C,Macri' E,Dal Cin E,Martignoni G,Doglioni C||Genes, chromosomes and cancer (46:419)||2007|
CDK-dependent activation of poly(ADP-ribose) polymerase member 10 (PARP10).
MA5-11732 was used in western blot to study the CDK-dependent activation of poly(ADP-ribose) polymerase member 10 (PARP10)
|Chou HY,Chou HT,Lee SC||The Journal of biological chemistry (281:15201)||2006|
Differential segregation and modification of mRNA during spermiogenesis in Marsilea vestita.
MA5-11732 was used in western blot to study the differential segregation and modification of mRNA during spermiogenesis in Marsilea vestita
|Tsai CW,Van Der Weele CM,Wolniak SM||Developmental biology (269:319)||2004|
Upregulation of tissue inhibitor of metalloproteinases (TIMP)-2 promotes matrix metalloproteinase (MMP)-2 activation and cell invasion in a human glioblastoma cell line.
MA5-11732 was used in western blot to study the role of TIMP-2 upregulation in promoting MMP2 activation and cell invasion in a human glioblastoma cell line
|Lu KV,Jong KA,Rajasekaran AK,Cloughesy TF,Mischel PS||Laboratory investigation; a journal of technical methods and pathology (84:8)||2004|
Altered levels and regulation of stathmin in paclitaxel-resistant ovarian cancer cells.
MA5-11732 was used in western blot to investigate the changes of stathmin in specific cancer cell lines
|Balachandran R,Welsh MJ,Day BW||Oncogene (22:8924)||2003|
The topoisomerase II-Hsp90 complex: a new chemotherapeutic target?
MA5-11732 was used in immunoprecipitation and western blot to study the topoisomerase II-Hsp90 complex as a potential new chemotherapeutic target
|Barker CR,Hamlett J,Pennington SR,Burrows F,Lundgren K,Lough R,Watson AJ,Jenkins JR||International journal of cancer (118:2685)||2006|