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Invitrogen
beta Tubulin Loading Control Monoclonal Antibody (BT7R)
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FIGURE: 1 / 36
beta Tubulin Loading Control Antibody (MA5-16308) in ICC/IF
Immunofluorescent analysis of Beta-Tubulin (red) in HEK293T cells. Cells fixed in 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a Beta-Tubulin monoclonal antibody (Product # MA5-16308) at a dilution of 1:100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and in... View More
Product Details
MA5-16308
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Human,
Mouse,
Non-human primate,
Rabbit,
Rat
Published species
Avian,
C. elegans,
Chicken,
Fruit fly,
Hamster,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
BT7R
Immunogen
KLH conjugated peptide of the N-terminus of Beta-Tubulin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: beta 5-tubulin; beta Ib tubulin; beta tubulin 1; beta-4 tubulin; beta-4-tubulin; beta-5 tubulin; beta-tubulin; Beta-tubulin class-I; Beta-tubulin class-II; beta-tubulin class-III; Beta-tubulin class-V; Beta-tubulin class-VI; beta-tubulin T beta15 (aa 1-445); betaIII-tubulin; c(beta)7 tubulin; class I beta-tubulin; class II beta-tubulin isotype; class IIa beta-tubulin; class IIb beta-tubulin; class III beta-tubulin; class IVa beta-tubulin; class V beta-tubulin; dJ40E16.7; dystonia 4, torsion (autosomal dominant); HSA10p15 beta-tubulin 4Q; M40 antibody; Neuron-specific class III beta-tubulin; T beta-15; tubb2a {ECO:0000250|UniProtKB:Q7TMM9}; Tubulin 5 beta; tubulin beta; Tubulin beta 4'; Tubulin beta 8 class VIII; Tubulin beta chain; Tubulin beta class IIa; Tubulin beta class V; tubulin beta MGC4083; Tubulin beta-1 chain; Tubulin beta-2 chain; Tubulin beta-2A chain; Tubulin beta-2B chain; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4A chain; Tubulin beta-5 chain; Tubulin beta-6 chain; Tubulin beta-7 chain; Tubulin beta-8 chain; Tubulin beta-III; tubulin, beta 1 class VI; tubulin, beta 2; tubulin, beta 2A; tubulin, beta 2A class IIa; tubulin, beta 2B class IIb; tubulin, beta 3; tubulin, beta 3 class III; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta 4A class IVa; tubulin, beta 6 class V; tubulin, beta class I; tubulin, beta polypeptide; tubulin, beta polypeptide 2; tubulin, beta polypeptide paralog; tubulin, beta, 5; unnamed protein product
Gene Aliases: 2310057H16Rik; 2410129E14Rik; 2810484G07Rik; 3200002H15Rik; AA408537; AI325297; AI596182; B130022C14Rik; bA506K6.1; bA631M21.2; BB220206; beta-4; beta-5; brdp; CDCBM; CDCBM1; CDCBM5; CDCBM6; CFEOM3; CFEOM3A; CSCSC1; DYT4; FEOM3; HsT1601; M(beta)1; M(beta)2; M(beta)3; M(beta)4; M(beta)5; M(beta)6; M40; OK/SW-cl.56; OOMD; OOMD2; PMGYSA; RGD1305887; RGD1309427; TUBB; TUBB-5; TUBB1; TUBB2; TUBB2A; TUBB2B; TUBB3; TUBB4; TUBB4A; TUBB5; TUBB6; TUBB8
UniProt ID: (Chicken) P09244, (Human) P07437, (Chicken) P09207, (Human) Q9H4B7, (Human) Q13885, (Human) A8K068, (Chicken) P32882, (Human) Q13509, (Human) P04350, (Human) Q9BUF5, (Chicken) P09653, (Human) Q3ZCM7, (Mouse) A2AQ07, (Rat) P85108, (Mouse) Q7TMM9, (Mouse) Q9CWF2, (Rat) Q3KRE8, (Mouse) Q9ERD7, (Rat) Q4QRB4, (Mouse) Q9D6F9, (Mouse) P99024, (Rat) P69897, (Mouse) Q922F4
Entrez Gene ID: (Rabbit) 100349792, (Chicken) 396254, (Dog) 474830, (Human) 203068, (Rabbit) 100358689, (Chicken) 396427, (Human) 81027, (Rabbit) 100347858, (Dog) 485950, (Human) 7280, (Chicken) 768337, (Dog) 478702, (Human) 347733, (Chicken) 420883, (Chicken) 431043, (Dog) 102154194, (Human) 10381, (Dog) 100855815, (Human) 10382, (Rabbit) 100348290, (Human) 84617, (Rabbit) 100350252, (Chicken) 421037, (Dog) 480213, (Human) 347688, (Mouse) 545486, (Rat) 679312, (Rat) 498736, (Mouse) 22151, (Mouse) 73710, (Rat) 291081, (Mouse) 22152, (Rat) 246118, (Rat) 29213, (Mouse) 22153, (Mouse) 22154, (Rat) 29214, (Mouse) 67951, (Rat) 307351
GTPase activity
structural constituent of cytoskeleton
GTP binding
ubiquitin protein ligase binding
MHC class I protein binding
structural molecule activity
protein binding
protein domain specific binding
protein complex binding
GTPase activating protein binding
calcium ion binding
peptide binding
molecular_function
nucleotide binding
tubulin
microtubule cytoskeleton organization
mitotic cell cycle
microtubule-based process
regulation of synapse organization
spindle assembly
G2/M transition of mitotic cell cycle
movement of cell or subcellular component
cellular process
cytoskeleton-dependent intracellular transport
natural killer cell mediated cytotoxicity
cell division
cytoskeleton organization
neuron migration
negative regulation of microtubule polymerization
axon guidance
neuron differentiation
mitotic nuclear division
biological_process
protein polymerization
metabolic process
cell projection organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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