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An siRNA targeting ß-actin was labeled with Cy™3 using the Silencer™ siRNA Labeling Kit. The labeled siRNA was transfected into HeLa cells and cells were analyzed 96 hours later. Green: ß-actin protein detected with anti-ß-actin (Ambion) and a fluorescein labeled secondary antibody. Red: Cy3 labeled siRNA. Blue: DAPI stained nuclei. (Cy™3 is a trademark of Amersham Biosciences.)
|Tested species reactivity||Bovine, Dog, Chicken, Fruit fly, Cat, Guinea pig, Human, Sheep, Pig, Rabbit|
|Published species reactivity||Bovine, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||A slightly modified synthetic beta-cytoplasmic actin N-terminal peptide,Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH|
|Contains||15mM sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bacillus anthracis spores germinate extracellularly at air-liquid interface in an in vitro lung model under serum-free conditions.
AM4302 was used in immunocytochemistry to study spore dissemination after lung exposure.
|Powell JD,Hutchison JR,Hess BM,Straub TM||Journal of applied microbiology (119:711)||2015|
Activation of Adenosine Monophosphate-Activated Protein Kinase Is an Additional Mechanism That Participates in Mediating Inhibitory Actions of Prostaglandin F2Alpha in Mature, but Not Developing, Bovine Corpora Lutea.
AM4302 was used in western blot to study the inhibitory action of prostaglandin F2alpha in bovine corpora lutea and its mechanism
|Bowdridge EC,Goravanahally MP,Inskeep EK,Flores JA||Biology of reproduction (93:null)||2015|
Safety and angiogenic effects of systemic gene delivery of a modified erythropoietin.
AM4302 was used in western blot to study the angiogenic potential of erythropoietin
|de Lucas Cerrillo AM,Bond WS,Rex TS||Gene therapy (22:365)||2015|
Cyclin C is a haploinsufficient tumour suppressor.
AM4302 was used in western blot to elucidate the regulation of Notch by Cyclin C.
|Li N,Fassl A,Chick J,Inuzuka H,Li X,Mansour MR,Liu L,Wang H,King B,Shaik S,Gutierrez A,Ordureau A,Otto T,Kreslavsky T,Baitsch L,Bury L,Meyer CA,Ke N,Mulry KA,Kluk MJ,Roy M,Kim S,Zhang X,Geng Y,Zagozdzon A,Jenkinson S,Gale RE,Linch DC,Zhao JJ,Mullighan CG,Harper JW,Aster JC,Aifantis I,von Boehmer H,Gygi SP,Wei W,Look AT,Sicinski P||Nature cell biology (16:1080)||2014|
ACTB; actin, beta; actin, beta, cytoplasmic; actin, cytoplasmic 1; Atcb; beta actin; beta cytoskeletal actin; beta-actin; cytoplasmic 1; gamma non-muscle actin; PS1TP5-binding protein 1
ACT-5; ACTB; actin; Bact; BOS_22932; BRWS1; PS1TP5BP1; RCJMB04_4h19