An siRNA targeting ß-actin was labeled with Cy™3 using the Silencer™ siRNA Labeling Kit. The labeled siRNA was transfected into HeLa cells and cells were analyzed 96 hours later. Green: ß-actin protein detected with anti-ß-actin (Ambion) and a fluorescein labeled secondary antibody. Red: Cy3 labeled siRNA. Blue: DAPI stained nuclei. (Cy™3 is a trademark of Amersham Biosciences.)
|Tested species reactivity||Bovine, Dog, Chicken, Fruit fly, Cat, Guinea pig, Human, Sheep, Pig, Rabbit|
|Published species reactivity||Bovine, Hamster, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||A slightly modified synthetic beta-cytoplasmic actin N-terminal peptide,Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH|
|Contains||15mM sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Histone H3.3 promotes IgV gene diversification by enhancing formation of AID-accessible single-stranded DNA.
AM4302 was used in western blot to study promotion of IgV gene diversification by enhancing formation of AID-accessible single-stranded DNA by histone H3.3
|Romanello M,Schiavone D,Frey A,Sale JE||The EMBO journal (35:1452)||2016|
|Not Applicable||Not Cited||
Parallel Regulation of von Hippel-Lindau Disease by pVHL-Mediated Degradation of B-Myb and Hypoxia-Inducible Factor ¿.
AM4302 was used in western blot to study pVHL-mediated degradation of B-Myb and hypoxia-inducible factor alpha by parallele regulation of von Hippel-Lindau disease
|Okumura F,Uematsu K,Byrne SD,Hirano M,Joo-Okumura A,Nishikimi A,Shuin T,Fukui Y,Nakatsukasa K,Kamura T||Molecular and cellular biology (36:1803)||2016|
A Syndromic Intellectual Disability Disorder Caused by Variants in TELO2, a Gene Encoding a Component of the TTT Complex.
AM4302 was used in western blot to elucidate variants in TELO2, a gene encoding a component of the TTT complex, causing a syndromic intellectual disability disorder
|You J,Sobreira NL,Gable DL,Jurgens J,Grange DK,Belnap N,Siniard A,Szelinger S,Schrauwen I,Richholt RF,Vallee SE,Dinulos MB,Valle D,Armanios M,Hoover-Fong J||American journal of human genetics (98:909)||2016|
|Not Applicable||Not Cited||
Bromodomain inhibition of the transcriptional coactivators CBP/EP300 as a therapeutic strategy to target the IRF4 network in multiple myeloma.
AM4302 was used in western blot to determine a therapeutic strategy to target the IRF4 network in multiple myeloma by using the bromodomain inhibition of the transcriptional coactivators CBP/EP300
|Conery AR,Centore RC,Neiss A,Keller PJ,Joshi S,Spillane KL,Sandy P,Hatton C,Pardo E,Zawadzke L,Bommi-Reddy A,Gascoigne KE,Bryant BM,Mertz JA,Sims RJ||eLife (5:null)||2016|
|Not Applicable||Not Cited||
Non-Specific Blocking of miR-17-5p Guide Strand in Triple Negative Breast Cancer Cells by Amplifying Passenger Strand Activity.
AM4302 was used in western blot to determine the mechanism for blocking of miR-17-5p guide strand in triple negative breast cancer cells
|Jin YY,Andrade J,Wickstrom E||PloS one (10:null)||2015|
Elevated ¿-synuclein caused by SNCA gene triplication impairs neuronal differentiation and maturation in Parkinson's patient-derived induced pluripotent stem cells.
AM4302 was used in western blot to research the role of increased alpha-synuclein due to SNCA gene triplication and its role in Parkinson stem cells
|Oliveira LM,Falomir-Lockhart LJ,Botelho MG,Lin KH,Wales P,Koch JC,Gerhardt E,Taschenberger H,Outeiro TF,Lingor P,Schüle B,Arndt-Jovin DJ,Jovin TM||Cell death and disease (6:null)||2015|
Activation of Adenosine Monophosphate-Activated Protein Kinase Is an Additional Mechanism That Participates in Mediating Inhibitory Actions of Prostaglandin F2Alpha in Mature, but Not Developing, Bovine Corpora Lutea.
AM4302 was used in western blot to study the inhibitory action of prostaglandin F2alpha in bovine corpora lutea and its mechanism
|Bowdridge EC,Goravanahally MP,Inskeep EK,Flores JA||Biology of reproduction (93:null)||2015|
Safety and angiogenic effects of systemic gene delivery of a modified erythropoietin.
AM4302 was used in western blot to study the angiogenic potential of erythropoietin
|de Lucas Cerrillo AM,Bond WS,Rex TS||Gene therapy (22:365)||2015|
Cyclin C is a haploinsufficient tumour suppressor.
AM4302 was used in western blot to elucidate the regulation of Notch by Cyclin C.
|Li N,Fassl A,Chick J,Inuzuka H,Li X,Mansour MR,Liu L,Wang H,King B,Shaik S,Gutierrez A,Ordureau A,Otto T,Kreslavsky T,Baitsch L,Bury L,Meyer CA,Ke N,Mulry KA,Kluk MJ,Roy M,Kim S,Zhang X,Geng Y,Zagozdzon A,Jenkinson S,Gale RE,Linch DC,Zhao JJ,Mullighan CG,Harper JW,Aster JC,Aifantis I,von Boehmer H,Gygi SP,Wei W,Look AT,Sicinski P||Nature cell biology (16:1080)||2014|
A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies.
AM4302 was used in western blot to investigate the TGFBR3-JUND-KRT5 regulatory circuit that is time and matrix-dependent in single breast epithelial cells and basal-like premalignancies
|Wang CC,Bajikar SS,Jamal L,Atkins KA,Janes KA||Nature cell biology (16:345)||2014|
Mutations in NONO lead to syndromic intellectual disability and inhibitory synaptic defects.
AM4302 was used in western blot to characterize NONO-deficient mice
|Mircsof D,Langouët M,Rio M,Moutton S,Siquier-Pernet K,Bole-Feysot C,Cagnard N,Nitschke P,Gaspar L,¿nidari¿ M,Alibeu O,Fritz AK,Wolfer DP,Schröter A,Bosshard G,Rudin M,Koester C,Crestani F,Seebeck P,Boddaert N,Prescott K,Hines R,Moss SJ,Fritschy JM,Munnich A,Amiel J,Brown SA,Tyagarajan SK,Colleaux L||Nature neuroscience (18:1731)||2015|
Impact of Diverse Immune Evasion Mechanisms of Cancer Cells on T Cells Engaged by EpCAM/CD3-Bispecific Antibody Construct AMG 110.
AM4302 was used in western blot to study the use of bispecific T cell engager
|Deisting W,Raum T,Kufer P,Baeuerle PA,Münz M||PloS one (10:null)||2015|
Deregulated Expression of SRC, LYN and CKB Kinases by DNA Methylation and Its Potential Role in Gastric Cancer Invasiveness and Metastasis.
AM4302 was used in western blot to assess SRC, LYN, and CKB expression, as well as their promoter methylation, in gastric cancer
|Mello AA,Leal MF,Rey JA,Pinto GR,Lamarão LM,Montenegro RC,Alves AP,Assumpção PP,Borges Bdo N,Smith MC,Burbano RR||PloS one (10:null)||2015|
The RNA-binding protein LIN28B regulates developmental timing in the mammalian cochlea.
AM4302 was used in western blot to elucidate the molecular mechanisms involved in the timing and coordination of auditory prosensory proliferation and differentiation.
|Golden EJ,Benito-Gonzalez A,Doetzlhofer A||Proceedings of the National Academy of Sciences of the United States of America (112:E3864)||2015|
An analysis of critical factors for quantitative immunoblotting.
AM4302 was used in western blot to examine an immunoblot-analysis workflow for accuracy and precision
|Janes KA||Science signaling (8:null)||2015|
Mesenchymal stem cells contribute to the chemoresistance of hepatocellular carcinoma cells in inflammatory environment by inducing autophagy.
AM4302 was used in western blot to examine the role of mesenchymal stem cells in the development of hepatocellular carcinoma resistance to chemotherapy.
|Han Z,Jing Y,Xia Y,Zhang S,Hou J,Meng Y,Yu F,Liu X,Wu M,Zhang P,Wei L||Cell and bioscience (4:null)||2014|
Serum stability and physicochemical characterization of a novel amphipathic peptide C6M1 for siRNA delivery.
AM4302 was used in western blot to investigate the use of peptides as carriers of short interfering RNA.
|Jafari M,Xu W,Pan R,Sweeting CM,Karunaratne DN,Chen P||PloS one (9:null)||2014|
Mechanisms of intracellular calcium homeostasis in developing and mature bovine corpora lutea.
AM4302 was used in western blot to study mechanisms of calcium homeostasis in bovine corpora lutea.
|Wright MF,Bowdridge E,McDermott EL,Richardson S,Scheidler J,Syed Q,Bush T,Inskeep EK,Flores JA||Biology of reproduction (90:null)||2014|
Tumor suppressor Nf2 limits expansion of the neural progenitor pool by inhibiting Yap/Taz transcriptional coactivators.
AM4302 was used in western blot to demonstrate that tumor suppressor neurofibromatosis 2 limits the expansion of neural progenitor cells.
|Lavado A,He Y,Paré J,Neale G,Olson EN,Giovannini M,Cao X||Development (Cambridge, England) (140:3323)||2013|
Spatial regulation of interleukin-6 signaling in response to neurodegenerative stressors in the retina.
AM4302 was used in western blot to study the effect of IL-6 on retinal ganglion cells.
|Sims SM,Holmgren L,Cathcart HM,Sappington RM||American journal of neurodegenerative disease (1:168)||2013|
Effect of cellular senescence on the albumin permeability of blood-derived endothelial cells.
AM4302 was used in western blot to study permeability and tight junctions in endothelial cells derived from human cord blood.
|Cheung TM,Ganatra MP,Peters EB,Truskey GA||American journal of physiology. Heart and circulatory physiology (303:H1374)||2012|
Moloney murine leukemia virus genomic RNA packaged in the absence of a full complement of wild type nucleocapsid protein.
AM4302 was used in western blot to study the role of Gag proteins in gRNA packaging.
|Johnson SF,Garcia EL,Summers MF,Telesnitsky A||Virology (430:100)||2012|
Transient aggregation of ubiquitinated proteins is a cytosolic unfolded protein response to inflammation and endoplasmic reticulum stress.
AM4302 was used in western blot to investigate the regulatory mechanisms of aggresome-like induced structures formation and clearance.
|Liu XD,Ko S,Xu Y,Fattah EA,Xiang Q,Jagannath C,Ishii T,Komatsu M,Eissa NT||The Journal of biological chemistry (287:19687)||2012|
Bacillus anthracis spores germinate extracellularly at air-liquid interface in an in vitro lung model under serum-free conditions.
AM4302 was used in immunocytochemistry to study spore dissemination after lung exposure.
|Powell JD,Hutchison JR,Hess BM,Straub TM||Journal of applied microbiology (119:711)||2015|