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Western blot analysis of Beta-Tubulin was performed by loading 50ug of various cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated Beta-Tubulin monoclonal antibody (Product # MA5-16308-HRP) at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
|Tested species reactivity||Chicken, Human, Mouse, Primate, Rabbit, Rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||KLH conjugated peptide of the N-terminus of Beta-Tubulin.|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-16308-HRP has been successfully used in Western blotting applications with human, mouse, rat, rabbit, non-human primate, and chicken samples.
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
beta 5-tubulin; beta Ib tubulin; c(beta)7 tubulin; TUBB; tubulin beta 4'; tubulin beta-1 chain; tubulin beta-5 chain; tubulin beta-7 chain; tubulin, beta 1 class VI; tubulin, beta 2; tubulin, beta 2A; tubulin, beta 2A class IIa; tubulin, beta class I; tubulin, beta polypeptide
2810484G07Rik; CDCBM6; CSCSC1; M(beta)1; M40; OK/SW-cl.56; TUBB; TUBB1; TUBB2A; TUBB5