Invitrogen

betatrophin Monoclonal Antibody (OTI1B12)

Product Image Gallery

betatrophin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min. Following antigen retrieval, tissues were probed with a C19ORF80 monoclonal antibody (Product # MA5-27043) at a dilution of 1:150.

betatrophin Antibody in Western Blot (WB) — Western blot analysis of C19ORF80 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with C19ORF80 (Product # MA5-27043) monoclonal antibody.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Western Blot (WB)

1:2000

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI1B12

Immunogen

Human recombinant protein fragment corresponding to amino acids 22-198 of C19ORF80 produced in E.coli

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2723975

Target Information

The Betatrophin protein, also known as the hepatocellular carcinoma-associated protein TD26, is an adipocyte- and liver-enriched insulin targeted gene that is thought to play a role in lipid metabolism be regulating serum triglyceride levels. Betatrophin is closely related to the angiopoietin-like (ANGPTL) protein family. Betatrophin mRNA increases in the cold environment and after treatment with a high-fat diet, while its mRNA levels decrease in response to fasting, suggesting that it may play a role in mediating thermogenesis and energy homeostasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: angiopoietin-like 8; Angiopoietin-like protein 8; ANGPTL8; Betatrophin; betatrophin variant 1; betatrophin variant 2; hepatocellular carcinoma-associated gene TD26; hepatocellular carcinoma-associated protein TD26; Lipasin; PRO1185; PVPA599; Refeeding-induced fat and liver protein; RIFL; TD26; UNQ599/PRO1185

Gene Aliases: ANGPTL8; C19orf80; PRO1185; PVPA599; RIFL; TD26; UNQ599/PRO1185

UniProt ID: (Human) Q6UXH0

Entrez Gene ID: (Human) 55908

Function(s)

positive regulation of protein processing regulation of lipid metabolic process cellular lipid metabolic process type B pancreatic cell proliferation fat cell differentiation cell maturation regulation of lipoprotein metabolic process triglyceride homeostasis

Process(es)

hormone activity protein binding