AbboMax

c-Abl Monoclonal Antibody (ABM328)

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Datasheet
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

500-6564

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-1 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

2-10 µg/mL
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

ABM328

Immunogen

The synthetic peptide corresponding to SH2 domain of c-Abl protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS with proprietary stabilizer

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

Positive control: Placenta or Hela cell

Cellular location: Cytoplasmic, nuclear.

Target Information

The ABL1 proto-oncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns c-Abl into an oncogene. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for c-Abl. In chronic myelogenous leukemia and a subset of acute lymphoblastic leukemias, the c-Abl proto oncogene undergoes a (9;22) chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome). As the result of the fusion of c-Abl sequences from chromosome 9 to the Bcr gene on chromosome 22. The c-Abl oncogene was initially identified as the viral transforming gene of Abelson murine leukemia virus (A-MuLV). c-ABL potentially regulates DNA repair by activating the proapoptotic pathway when the DNA damage is too severe to be repaired.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Abelson murine leukemia oncogene; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Abl 1 antib; ABL2; ABLL; ARG; B30; bcr/c-abl oncogene protein; c-abl oncogene 1, receptor tyrosine kinase; p150; Proto-oncogene c-Abl; proto-oncogene tyrosine-protein kinase ABL1; RP11-83J21.1; Tyrosine kinase ARG; Tyrosine-protein kinase ABL1; v-abl Abelson murine leukemia oncogene 1; v-abl Abelson murine leukemia viral oncogene homolog 1

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Gene Aliases: ABL; ABL1; AI325092; bcr/abl; c-ABL; c-ABL1; E430008G22Rik; JTK7; p150; v-abl

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UniProt ID: (Human) P00519, (Mouse) P00520

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Entrez Gene ID: (Human) 25, (Mouse) 11350

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Function(s)
magnesium ion binding DNA binding actin monomer binding nicotinate-nucleotide adenylyltransferase activity protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein kinase C binding receptor binding protein binding ATP binding protein C-terminus binding SH3 domain binding syntaxin binding manganese ion binding actin filament binding mitogen-activated protein kinase binding proline-rich region binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding protein domain specific binding metal ion binding delta-catenin binding
Process(es)
B-1 B cell homeostasis positive regulation of protein phosphorylation B cell proliferation involved in immune response transitional one stage B cell differentiation mismatch repair regulation of transcription, DNA-templated cellular protein modification process autophagy cellular response to DNA damage stimulus DNA damage induced protein phosphorylation response to oxidative stress cell cycle arrest mitotic nuclear division epidermal growth factor receptor signaling pathway positive regulation of cytosolic calcium ion concentration intrinsic apoptotic signaling pathway in response to DNA damage regulation of autophagy peptidyl-tyrosine phosphorylation cerebellum morphogenesis negative regulation of cell-cell adhesion microspike assembly actin cytoskeleton organization regulation of endocytosis regulation of cell adhesion negative regulation of BMP signaling pathway regulation of axon extension regulation of microtubule polymerization regulation of actin cytoskeleton organization positive regulation of osteoblast proliferation substrate adhesion-dependent cell spreading cellular response to oxidative stress platelet-derived growth factor receptor-beta signaling pathway peptidyl-tyrosine autophosphorylation Fc-gamma receptor signaling pathway involved in phagocytosis regulation of cell proliferation signal transduction in response to DNA damage positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling innate immune response establishment of protein localization negative regulation of mitotic cell cycle positive regulation of mitotic cell cycle alpha-beta T cell differentiation protein autophosphorylation spleen development thymus development collateral sprouting positive regulation of peptidyl-tyrosine phosphorylation activated T cell proliferation B cell receptor signaling pathway neuromuscular process controlling balance positive regulation of muscle cell differentiation positive regulation of release of sequestered calcium ion into cytosol positive regulation of oxidoreductase activity negative regulation of ubiquitin-protein transferase activity mitochondrial depolarization Bergmann glial cell differentiation cellular response to hydrogen peroxide negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide negative regulation of protein serine/threonine kinase activity actin filament branching positive regulation of interleukin-2 secretion negative regulation of phospholipase C activity positive regulation of neuron death positive regulation of interferon-gamma secretion regulation of extracellular matrix organization cellular response to dopamine positive regulation of microtubule binding positive regulation of actin filament binding activation of protein kinase C activity positive regulation of Wnt signaling pathway, planar cell polarity pathway regulation of cell motility regulation of actin cytoskeleton reorganization negative regulation of endothelial cell apoptotic process negative regulation of cellular senescence regulation of response to DNA damage stimulus DNA repair protein phosphorylation endocytosis phagocytosis apoptotic process cell adhesion phosphorylation cell migration B cell proliferation platelet-derived growth factor receptor signaling pathway regulation of cell cycle regulation of cellular senescence
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