|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Synthetic peptide: KVEQLSPEEEEKRRIRRERNKMAAA, corresponding to Internal sequence amino acids 128 - 152 of Human c-Fos|
|Storage buffer||0.05M sodium phosphate, pH 7.5, with 0.2% gelatin|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2.5-5.0 µg/ml.|
|Western Blot (WB)||2.5-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-21190 detects c-Fos in human, mouse, and rat samples. This antibody does not cross-react with the 39 kDa jun protein.
MA1-21190 has been successfully used in immunocytochemistry and Western blot procedures. By Western blot, this antibody detects ~55 and 62 kDa bands representing c-fos and v-fos, respectively. MA1-21190 is not suitable for IHC applications on frozen or paraffin tissues. Positive control of HeLa Cells suggested.
The MA1-21190 immunogen is a synthetic peptide: KVEQLSPEEEEKRRIRRERNKMAAA corresponding to Internal sequence amino acids 128 - 152 of Human c-Fos.
c-FOS is a nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. It has a critical function in regulating the development of cells destined to form and maintain the skeleton, and is thought to have an important role in signal transduction, cell proliferation and differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.