Invitrogen

c-Jun Monoclonal Antibody (5B1)

2 Published Figures

View all (139) c-Jun antibodies

Questions & Answers

Questions & Answers

Product Image Gallery

c-Jun Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PC-2 cells using c-Jun monoclonal antibody (Product # MA5-15881) (Green). Red: actin filaments have been labeled with phalloidin.

c-Jun Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of paraffin-embedded human Tunica intima cancer tissues using c-Jun monoclonal antibody (Product # MA5-15881) followed with DAB staining.

c-Jun Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using c-Jun monoclonal antibody (Product # MA5-15881) followed with DAB staining.

c-Jun Antibody in Western Blot (WB) — Western blot analysis of c-Jun using a c-Jun monoclonal antibody (Product # MA5-15881) against a human c-Jun (AA: 199-331) recombinant protein.

c-Jun Antibody in Western Blot (WB) — Western blot analysis of c-Jun using c-Jun monoclonal antibody (Product # MA5-15881) in NIH/3T3 (1) and COS-7 (2) cell lysate.

c-Jun Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of HepG2 cells using c-Jun monoclonal antibody (Product # MA5-15881) (blue) and negative control (red).

c-Jun Antibody in Western Blot (WB) — Figure 2 Analysis of E2 signal transduction. ( a ) cAMP assay showing cAMP levels (nM) in MCF-10A cells following treatment with 32 nM E2 for 15 min, 30 min, 24 h, and 48 h. Three independent experiments were performed. Bars represent +/-SD. ( b ) Western blotting of MCF-10A cells showing p38 and phospho-p38 (Thr180/Tyr182) following treatment with 32 nM E2 for 0-60 min. ( c ) Western blotting of MCF-10A cells treated with 32 nM E2 (left panel) or with 32 nM E2 and 20 nM G-15 (right panel) for 0-30 min. ( d ) Western blotting of MCF-10A cells showing JNK and phosphor-JNK (Thr183/Tyr185) following treatment with 32 nM E2 for 0-60 min. ( e ) Western blotting of MCF-10A cells treated with 32 nM E2 (left panel) or with 32 nM E2 and 20 nM G-15 (right panel) for 0-30 min. ( f ) Western blotting of MCF-10A cells treated with 32 nM E2 for 0-60 min showing IkB and phospho-IkB (Ser32, Ser36). ( g ) Western blotting of MCF-10A cells treated with 32 nM E2 for 0-60 min showing c-Jun and phospho-c-Jun (Ser63). ( h ) Representative confocal images of Accell siRNA-GPER- or siRNA-control-transfected MCF-10A cells in a 3D culture through the middle acini, which were treated with E2 (32 nM, left panels) or control (0 nM, right panel) for 7 days. Laminin V (red); pan-cadherin (green). Scale bars = 20 mum. The presented blots were cropped. Full-length blots are presented in Supplementary Fig. 5 .

c-Jun Antibody in Western Blot (WB) — Published figure using c-Jun monoclonal antibody (Product # MA5-15881) in Western Blot

Product Details

MA5-15881

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 2 publications 2 publications
Immunohistochemistry (IHC)	1:200-1:1,000	-
Immunocytochemistry (ICC/IF)	1:200-1:1,000	-
Flow Cytometry (Flow)	1/200 - 1/400	-
ELISA (ELISA)	1:10,000	-

Product Specifications
Species Reactivity	Human, Mouse, Non-human primate
Published species	Not Applicable
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	5B1
Immunogen	Purified recombinant fragment of human c-Jun expressed in E. Coli.
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	ascites
Contains	0.03% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11153582

Product Specific Information

MA5-15881 targets c-Jun in FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, and Non-human primate samples.

The MA5-15881 immunogen is purified recombinant fragment of human c-Jun expressed in E. Coli. .

MA5-15881 detects c-Jun which has a predicted molecular weight of approximately 43kDa.

Target Information

c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Estradiol/GPER affects the integrity of mammary duct-like structures in vitro.

Scientific reports

Deng Y,Miki Y,Nakanishi A

Published figure using c-Jun monoclonal antibody (Product # MA5-15881) in Western Blot

Tue Jan 28 00:00:00 EST 2020

C6orf106 is a novel inhibitor of the interferon-regulatory factor 3-dependent innate antiviral response.

The Journal of biological chemistry

Ambrose RL,Liu YC,Adams TE,Bean AGD,Stewart CR

Published figure using c-Jun monoclonal antibody (Product # MA5-15881) in Western Blot

Fri Jul 06 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Activator protein 1; AH119; AP1; cjun; enhancer-binding protein AP1; immediate early; Jun A; Jun activation domain binding protein; Jun oncogene; jun proto-oncogene; JUND; p39; Proto-oncogene c-Jun; Transcription factor AP-1; V-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog

Gene Aliases: AP-1; AP1; c-Jun; JUN; Junc

UniProt ID: (Human) P05412, (Mouse) P05627

Entrez Gene ID: (Human) 3725, (Mouse) 16476

Molecular Function: basic leucine zipper transcription factor

angiogenesis microglial cell activation release of cytochrome c from mitochondria liver development positive regulation of endothelial cell proliferation outflow tract morphogenesis transcription from RNA polymerase II promoter transforming growth factor beta receptor signaling pathway SMAD protein import into nucleus Ras protein signal transduction aging learning circadian rhythm negative regulation of cell proliferation response to radiation response to mechanical stimulus positive regulation of epithelial cell migration regulation of cell death monocyte differentiation axon regeneration negative regulation of protein autophosphorylation response to lipopolysaccharide cellular response to hormone stimulus response to cytokine leading edge cell differentiation response to muscle stretch Fc-epsilon receptor signaling pathway regulation of cell proliferation response to drug response to hydrogen peroxide negative regulation of DNA binding negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process positive regulation of GTPase activity negative regulation by host of viral transcription positive regulation by host of viral transcription positive regulation of cell differentiation positive regulation of monocyte differentiation positive regulation of DNA replication negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation positive regulation of smooth muscle cell proliferation regulation of sequence-specific DNA binding transcription factor activity cellular response to potassium ion starvation response to cAMP regulation of cell cycle membrane depolarization SMAD protein signal transduction eyelid development in camera-type eye positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress positive regulation of DNA-templated transcription, initiation negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated positive regulation of cell proliferation cellular process positive regulation of apoptotic process negative regulation of apoptotic process

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity GTPase activator activity protein binding transcription factor binding enzyme binding cAMP response element binding identical protein binding protein homodimerization activity transcription regulatory region DNA binding poly(A) RNA binding protein heterodimerization activity R-SMAD binding HMG box domain binding double-stranded DNA binding sequence-specific DNA binding