Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2,000 | View 2 publications 2 publications |
Immunohistochemistry (IHC) |
1:200-1:1,000 | - |
Immunocytochemistry (ICC/IF) |
1:200-1:1,000 | - |
Flow Cytometry (Flow) |
1/200 - 1/400 | - |
ELISA (ELISA) |
1:10,000 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Non-human primate |
Published species |
Not Applicable |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
5B1 |
Immunogen |
Purified recombinant fragment of human c-Jun expressed in E. Coli. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
Conc. Not Determined |
Storage buffer |
ascites |
Contains |
0.03% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_11153582 |
MA5-15881 targets c-Jun in FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, and Non-human primate samples.
The MA5-15881 immunogen is purified recombinant fragment of human c-Jun expressed in E. Coli. .
MA5-15881 detects c-Jun which has a predicted molecular weight of approximately 43kDa.
c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Activator protein 1; AH119; AP1; cjun; enhancer-binding protein AP1; immediate early; Jun A; Jun activation domain binding protein; Jun oncogene; jun proto-oncogene; JUND; p39; Proto-oncogene c-Jun; Transcription factor AP-1; V-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog
Gene Aliases: AP-1; AP1; c-Jun; JUN; Junc
UniProt ID: (Human) P05412, (Mouse) P05627
Entrez Gene ID: (Human) 3725, (Mouse) 16476
Molecular Function:
basic leucine zipper transcription factor
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