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Staining of C57Bl/6 bone marrow cells with Mouse Hematopoietic Lineage eFluor® 450 Cocktail, Anti-Mouse Ly-6A/E (Sca-1) PerCP-Cyanine5.5, and 0.06 ug of Anti-Mouse CD117 (c-Kit) PE (A16395). Lineage negative/low, viable cells, as determined by Fixable Viability Dye eFluor® 780, were used for analysis.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2b, kappa|
|Immunogen||IL-3-dependent mast cells derived from normal bone marrow.|
|Storage buffer||proprietary buffer|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
c-Kit, also known as CD117 and stem cell factor receptor, is a 145 kDa transmembrane tyrosine kinase encoded by the c-Kit proto-oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. c-Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer. Phosphorylation at tyrosine 703 of c-Kit allows binding of Grb2 and activation of the Ras-Raf-ERK1 and 2 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Hematopoiesis and stem cell renewal in long-term bone marrow cultures containing catalase.
A16395 was used in flow cytometry to investigate how peroxide affects hematopoiesis
|Gupta R,Karpatkin S,Basch RS||Blood (107:1837)||2006|
belly-spot; C-Kit; c-kit proto-oncogene protein; CD117; ckit; dominant spotting; Dominant white spotting; Mast/stem cell growth factor receptor Kit; piebald trait protein; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; spotted sterile male; Steel Factor Receptor; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
Bs; c-KIT; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; Kit; SCO1; SCO5; Sl; SOW3; Ssm; Tr-kit; W