|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||IL-3 dependent murine mast cells|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.1 - 0.2 µg/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-70079 detects CD117 in mouse samples.
MA1-70079 has been successfully used in flow cytometry procedures.
The MA1-70079 immunogen is IL-3 dependent mouse mast cells.
Store at 4°C. For long term storage, store at -20°C in single use aliquots, avoiding freeze/thaw cycles.
c-Kit, also known as CD117 and stem cell factor receptor, is a 145 kDa transmembrane tyrosine kinase encoded by the c-Kit proto-oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. c-Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer. Phosphorylation at tyrosine 703 of c-Kit allows binding of Grb2 and activation of the Ras-Raf-ERK1 and 2 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Consequences of the combined loss of BOK and BAK or BOK and BAX.
MA1-70079 was used in flow cytometry to determine if Bok and Bax have any functional redundancy controlling apoptosis using knock out mice.
|Ke F,Bouillet P,Kaufmann T,Strasser A,Kerr J,Voss AK||Cell death and disease (4:null)||2013|
Distinct osteoclast precursors in the bone marrow and extramedullary organs characterized by responsiveness to Toll-like receptor ligands and TNF-alpha.
MA1-70079 was used in blocking/activating experiment to study the role of TLRs ligands and tumor necross factor-alpha in osteoclast precursors in bone marrow and extramedullary organs
|Hayashi S,Yamada T,Tsuneto M,Yamane T,Takahashi M,Shultz LD,Yamazaki H||Journal of immunology (Baltimore, Md. : 1950) (171:5130)||2003|