Immunofluorescence analysis of c-Kit / CD117 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled c-Kit / CD117 (K45) Mouse Monoclonal Antibody (MA512944) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic and membranous localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Pig, Mouse, Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant human extracellular c-kit fragment|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12944 targets CD117 in IF/ICC and IHC (P, F) applications and shows reactivity with Human samples.
The MA5-12944 immunogen is recombinant human extracellular c-kit fragment.
c-kit is a transmembrane receptor tyrosine kinase. It binds to stem cell factor (SCF). It is expressed in many tissues and cells. C-kit/SCf is involved in development of several lineages of stem cells, such as germ cells, neural crest-derived melanocytes and hematopoietic precursor cells Ab-3 does not interfere with the binding of SCF to c-kit
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
LRIG1 Regulates Ontogeny of Smooth Muscle-Derived Subsets of Interstitial Cells of Cajal in Mice.
MA5-12944 was used in immunohistochemistry to test if LRIG1 contributes to the ontogeny of interstitial cells of Cajal in the small and large intestines.
|Kondo J,Powell AE,Wang Y,Musser MA,Southard-Smith EM,Franklin JL,Coffey RJ||Gastroenterology (149:407)||2015|
Wharton's jelly-derived mesenchymal stem cells promote myocardial regeneration and cardiac repair after miniswine acute myocardial infarction.
MA5-12944 was used in immunohistochemistry to use a miniswine model of acute myocardial infarction to study the therapeutic effects of Wharton's jelly-derived mesenchymal stem cells on cardiac repair and performance
|Zhang W,Liu XC,Yang L,Zhu DL,Zhang YD,Chen Y,Zhang HY||Coronary artery disease (24:549)||2013|
Generation of functional blood vessels from a single c-kit+ adult vascular endothelial stem cell.
MA5-12944 was used in immunohistochemistry to study the ability of a single c-Kit+ adult vascular endothelial stem cell to give rise to functional blood vessels
|Fang S,Wei J,Pentinmikko N,Leinonen H,Salven P||PLoS biology (10:null)||2012|
Loss of Lrig1 leads to expansion of Brunner glands followed by duodenal adenomas with gastric metaplasia.
MA5-12944 was used in western blot to study duodenal adenomas in leucine-rich repeats and immunoglobulin-like domains 1 -/- mice
|Wang Y,Shi C,Lu Y,Poulin EJ,Franklin JL,Coffey RJ||The American journal of pathology (185:1123)||2015|
Human adult craniofacial muscle-derived cells: neural-cell adhesion-molecule (NCAM; CD56)-expressing cells appear to contain multipotential stem cells.
MA5-12944 was used in immunocytochemistry to characterize muscle-derived multipotential stem cells
|Sinanan AC,Hunt NP,Lewis MP||Biotechnology and applied biochemistry (40:25)||2004|