Immunofluorescence analysis of c-Kit / CD117 was performed using 70% confluent log phase Ramos cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with c-Kit / CD117 Rabbit Polyclonal Antibody (PA5-16458) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from C-terminus of human CD117/c-kit protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA5-16458 targets CD117 in IHC (P) applications and shows reactivity with Human samples.
The PA5-16458 immunogen is a synthetic peptide from C-terminus of human CD117/c-kit protein.
c-kit is a transmembrane receptor tyrosine kinase. It binds to stem cell factor (SCF). It is expressed in many tissues and cells. C-kit/SCf is involved in development of several lineages of stem cells, such as germ cells, neural crest-derived melanocytes and hematopoietic precursor cells Antibody to CD117/c-kit may be helpful in the histopathological identification of gastrointestinal stromal tumors (GIST).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Immunohistochemical clue for the histological overlap of salivary adenoid cystic carcinoma and polymorphous low-grade adenocarcinoma.
PA5-16458 was used in immunohistochemistry to study the potential of immunohistochemistry to distinguish alivary adenoid cystic carcinoma and polymorphous low-grade adenocarcinoma
|El-Nagdy S,Salama NM,Mourad MI||Interventional medicine and applied science (5:131)||2013|