Immunofluorescent analysis of CD117 (green) showing staining in the nucleus and cytoplasm of U-87 MG cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD117 polyclonal antibody (Product # PA5-16770) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Dog, Human, Rat|
|Published species reactivity||Pig, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from the cytoplasmic domain of human CD117 / c-kit protein|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Western Blot (WB)||1:50-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16770 targets CD117 in WB, IHC (P), FACS and ICC/IF applications and shows reactivity with Canine, Rat and Human samples.
The PA5-16770 immunogen is a synthetic peptide from the cytoplasmic domain of human CD117 / c-kit protein.
Recognizes a protein of 145kDa, which is identified as CD117/p145kit. This rabbit polyclonal antibody does not interfere with the binding of SCF to c-kit. It precipitates both the unoccupied as well as the occupied form of c-kit. The binding of the stem cell factor (SCF) to the c-kit-encoded receptor tyrosine kinase (Type III) stimulates a variety of biochemical responses that culminate in cellular proliferation, migration, or survival. C-kit plays an important role in hematopoiesis, melanogenesis, and gametogenesis. This antibody is excellent for staining of formalin-fixed, paraffin-embedded tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Phase 2 trial of dasatinib in target-selected patients with recurrent glioblastoma (RTOG 0627).
PA5-16770 was used in immunohistochemistry - paraffin section to run phase 2 trial of dasatinib in target-selected patients with recurrent glioblastoma
|Lassman AB,Pugh SL,Gilbert MR,Aldape KD,Geinoz S,Beumer JH,Christner SM,Komaki R,DeAngelis LM,Gaur R,Youssef E,Wagner H,Won M,Mehta MP||Neuro-oncology (17:992)||2015|
Wharton's jelly-derived mesenchymal stem cells promote myocardial regeneration and cardiac repair after miniswine acute myocardial infarction.
PA5-16770 was used in immunohistochemistry to use a miniswine model of acute myocardial infarction to study the therapeutic effects of Wharton's jelly-derived mesenchymal stem cells on cardiac repair and performance
|Zhang W,Liu XC,Yang L,Zhu DL,Zhang YD,Chen Y,Zhang HY||Coronary artery disease (24:549)||2013|