Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescent analysis of CD117 (green) showing staining in the nucleus and cytoplasm of U-87 MG cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD117 polyclonal antibody (Product # PA5-16770) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Dog, Human, Rat|
|Published species reactivity||Pig, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from the cytoplasmic domain of human CD117 / c-kit protein|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Western Blot (WB)||1:50-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16770 targets CD117 in WB, IHC (P), FACS and ICC/IF applications and shows reactivity with Canine, Rat and Human samples.
The PA5-16770 immunogen is a synthetic peptide from the cytoplasmic domain of human CD117 / c-kit protein.
Recognizes a protein of 145kDa, which is identified as CD117/p145kit. This rabbit polyclonal antibody does not interfere with the binding of SCF to c-kit. It precipitates both the unoccupied as well as the occupied form of c-kit. The binding of the stem cell factor (SCF) to the c-kit-encoded receptor tyrosine kinase (Type III) stimulates a variety of biochemical responses that culminate in cellular proliferation, migration, or survival. C-kit plays an important role in hematopoiesis, melanogenesis, and gametogenesis. This antibody is excellent for staining of formalin-fixed, paraffin-embedded tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Phase 2 trial of dasatinib in target-selected patients with recurrent glioblastoma (RTOG 0627).
PA5-16770 was used in immunohistochemistry - paraffin section to run phase 2 trial of dasatinib in target-selected patients with recurrent glioblastoma
|Lassman AB,Pugh SL,Gilbert MR,Aldape KD,Geinoz S,Beumer JH,Christner SM,Komaki R,DeAngelis LM,Gaur R,Youssef E,Wagner H,Won M,Mehta MP||Neuro-oncology (17:992)||2015|
Wharton's jelly-derived mesenchymal stem cells promote myocardial regeneration and cardiac repair after miniswine acute myocardial infarction.
PA5-16770 was used in immunohistochemistry to use a miniswine model of acute myocardial infarction to study the therapeutic effects of Wharton's jelly-derived mesenchymal stem cells on cardiac repair and performance
|Zhang W,Liu XC,Yang L,Zhu DL,Zhang YD,Chen Y,Zhang HY||Coronary artery disease (24:549)||2013|
C-Kit; c-KIT gene1; CD117; ckit; mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; piebald trait protein; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; receptor tyrosine kinase c-kit; SCFR; soluble KIT variant 1; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
C-Kit; CD117; KIT; PBT; SCFR