|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide VDTRPASFWETS , corresponding to C terminal amino acids 1379-1390 of Human Met (c-Met)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Neat|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29042 detects c-Met from human samples.
PA1-29042 has been successfully used in immunohistochemistry (paraffin tissue) applications. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Boil tissues in 10 mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 minutes. Suggested positive controls for this product are A431 cells, human breast carcinoma and human ovarian carcinoma.
The PA1-29042 immunogen is synthetic peptide: VDTRPASFWETS , corresponding to C terminal amino acids 1379-1390 of Human Met (c-Met).
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.