This Antibody was verified by IP Mass Spectrometry to ensure that the antibody binds to the antigen stated. View Details
This antibody is specific for the cytoplasmic domain of the c-Met protein. On western blotting, this antibody recognizes a band at approximately 145 kDa. During development reactivity was confirmed with human HT-29 breast carcinoma lysates and c-Met cytoplasmic domain recombinant protein by both western blot, and with formalin-fixed, paraffin-embedded breast carcinoma tissues by immunohistochemistry.
For immunohistochemistry with formalin-fixed, paraffin-embedded tissues, heat-induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining. Recommended antibody incubation period is overnight at 4°C.
The c-Met oncogene was originally isolated from a chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a transmembrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. The c-Met gene product has been identified as the cell surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration. c-Met, also known as HGF receptor or SF receptor, is activated by hepatocyte growth factor (HGF) or scatter factor (SF), and is composed of alpha and beta subunits linked by disulfide bonds. The alpha subunit of c-Met is extracellular and heavily glycosylated; the beta subunit contains an extracellular portion involved in ligand binding, a transmembrane segment, and a cytoplasmic tyrosine kinase domain. The truncated cytoplasmic region of c-Met has constitutive kinase activity and is oncogenic, but requires the first 39 amino acids of the juxtamembrane domain and the regulatory tyrosine in the catalytic domain to enact its transforming potential.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: c-met; EC 22.214.171.124; Hepatocyte growth factor receptor; Hepatocyte growth factor receptor precursor; HGF receptor; HGF-SF receptor; HGF/SF receptor; kinase EC 126.96.36.199; Met proto- oncogene tyros; Met proto- oncogene tyrosine kinase; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met
Gene Aliases: AUTS9; c-Met; DFNB97; HGFR; MET; RCCP2
UniProt ID: (Human) P08581
Entrez Gene ID: (Human) 4233