Invitrogen

c-Met Monoclonal Antibody (K.845.5)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. View Details

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Datasheet

Questions & Answers

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c-Met Antibody in Western Blot (WB) — Knockout of c-MET was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042) (Assay ID CRISPR752450_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of c-MET was performed by loading 30 µg of HeLa wild type (Lane 1), HeLa CAS9 (Lane 2), HeLa c-MET KO (Lane 3) membrane extracts. The blot was probed with Anti-c-Met Monoclonal Antibody (K.845.5)(Product # MA5-15198) using 1:1000 dilution and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to c-MET.

c-Met Antibody in Knockout — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in c-MET KO cell line compared to control cell line using Anti-c-Met Monoclonal Antibody (K.845.5)(Product # MA5-15198). {KO}

c-Met Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. A549 cells were transfected with siRNA against MET and decrease in signal intensity was observed in western blot application using c-Met Monoclonal Antibody (Product # MA5-15198). {KD}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

K.845.5

Immunogen

Synthetic peptide corresponding to residues surrounding Tyr1234 of human Met

Conjugate

Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

RRID

AB_10982997

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

MET is a tyrosine kinase receptor for the hepatocyte growth factor. It is linked to functions such as cell proliferation, scattering, morphogenesis, and survival. Ligand binding at the cell surface induces autophosphorylation of MET that provides docking sites for downstream signaling molecules. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: c-Met; cMet; EC 2.7.10.1; Hepatocyte growth factor receptor; HGF receptor; HGF-SF receptor; HGF/SF receptor; met proto-oncogene tyrosine kinase; oncogene MET; Proto-oncogene c-Met; sc MET; Scatter factor receptor; SF receptor; soluble c met; Tyrosine-protein kinase Met

Gene Aliases: AI838057; AUTS9; c-Met; DFNB97; HGF; HGFR; MET; Par4; RCCP2

UniProt ID: (Human) Q9UDX7, (Rat) P97523

Entrez Gene ID: (Human) 4233, (Rat) 24553, (Mouse) 17295

Function(s)