|Tested species reactivity||Human|
|Published species reactivity||Yeast, Bovine, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of human c-myc|
|Storage buffer||PBS, pH 7.4, with proprietary stabilizer|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1/100 - 1/500|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||1/100 - 1/500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This product does not require protein digestion or antigen retrieval prior to staining of paraffin sections. Non-reducing conditions are recommended for Western blot applications.
c-myc is a 62kDa protein involved in the regulation of cell cycles and cell growth. p62c-myc is primarily located to the cell nucleus, but has also been shown to localized to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Identification of two novel Shank3 transcripts in the developing mouse neocortex.
MA1-81357 was used in immunohistochemistry to study the role in the developing murine neocortex of two novel transcripts of the Shank3 synaptic scaffold protein
|Waga C,Asano H,Sanagi T,Suzuki E,Nakamura Y,Tsuchiya A,Itoh M,Goto Y,Kohsaka S,Uchino S||Journal of neurochemistry (128:280)||2014|
Differential expression of MYC in H. pylori-related intestinal and diffuse gastric tumors.
MA1-81357 was used in immunohistochemistry to characterize the expression of cell cycle proteins in Helicobacter-associated gastric cancer
|Silva-Fernandes IJ,Alves MK,Lima VP,de Lima MA,Barros MA,Ferreira MV,Rabenhorst SH||Virchows Archiv : an international journal of pathology (458:725)||2011|
Relationship between EBV infection and expression of cellular proteins c-Myc, Bcl-2, and Bax in gastric carcinomas.
MA1-81357 was used in immunohistochemistry to study the relationship between EBV infection and expression of c-Myc, Bcl-2, and Bax in gastric carcinomas
|Lima MA,Ferreira MV,Barros MA,Pardini MI,Ferrasi AC,Mota RM,Rabenhorst SH||Diagnostic molecular pathology : the American journal of surgical pathology, part B (17:82)||2008|
MAPK Hog1 closes the S. cerevisiae glycerol channel Fps1 by phosphorylating and displacing its positive regulators.
MA1-81357 was used in immunoprecipitation and western blot to study the regulation of the Saccharomyces cerevisiae Fps1 glycerol channel by Hog1-mediated phosphorylation
|Lee J,Reiter W,Dohnal I,Gregori C,Beese-Sims S,Kuchler K,Ammerer G,Levin DE||Genes and development (27:2590)||2013|
WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase.
MA1-81357 was used in immunoprecipitation to study the role of the WD40 protein FBW5 in promoting the ubiquitinylation of tumor suppresor protein TSC2
|Hu J,Zacharek S,He YJ,Lee H,Shumway S,Duronio RJ,Xiong Y||Genes and development (22:866)||2008|
ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity.
MA1-81357 was used in immunoprecipitation to investigate the interaction between ROC1 and cullin proteins
|Ohta T,Michel JJ,Schottelius AJ,Xiong Y||Molecular cell (3:535)||1999|
SNAP receptors implicated in vesicle targeting and fusion.
MA1-81357 was used in immunoprecipitation to characterize SNAP receptors isolated from bovine brain
|Söllner T,Whiteheart SW,Brunner M,Erdjument-Bromage H,Geromanos S,Tempst P,Rothman JE||Nature (362:318)||1993|
Fulvestrant induces resistance by modulating GPER and CDK6 expression: implication of methyltransferases, deacetylases and the hSWI/SNF chromatin remodelling complex.
MA1-81357 was used in western blot to study the roles of GPER and CDK6 in the mechanisms of fulvestrant resistance in ER-positive breast cancer cells
|Giessrigl B,Schmidt WM,Kalipciyan M,Jeitler M,Bilban M,Gollinger M,Krieger S,Jäger W,Mader RM,Krupitza G||British journal of cancer (109:2751)||2013|
Functional impact of Aurora A-mediated phosphorylation of HP1¿ at serine 83 during cell cycle progression.
MA1-81357 was used in western blot to study the cell cycle role of HP1-gamma phosphorylation at Ser 83 by Aurora A
|Grzenda A,Leonard P,Seo S,Mathison AJ,Urrutia G,Calvo E,Iovanna J,Urrutia R,Lomberk G||Epigenetics and chromatin (6:null)||2013|
Activation of a PGC-1-related coactivator (PRC)-dependent inflammatory stress program linked to apoptosis and premature senescence.
MA1-81357 was used in western blot to study the activation of the PRC-dependent stress program by apoptosis and senescence and its role in the response to cellular dysfunction
|Gleyzer N,Scarpulla RC||The Journal of biological chemistry (288:8004)||2013|
|Not Applicable||Not Cited||
The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A.
MA1-81357 was used in western blot to study the role of Aurora A kinase in the mechanism by which CUL3-KLHL18 regulates cell cycle entry
|Moghe S,Jiang F,Miura Y,Cerny RL,Tsai MY,Furukawa M||Biology open (1:82)||2012|
Regulation of fertility, survival, and cuticle collagen function by the Caenorhabditis elegans eaf-1 and ell-1 genes.
MA1-81357 was used in western blot to study the in vivo functions of the EAF2 and ELL family proteins in Caenorhabditis elegans
|Cai L,Phong BL,Fisher AL,Wang Z||The Journal of biological chemistry (286:35915)||2011|
Selective interaction between Trf3 and Taf3 required for early development and hematopoiesis.
MA1-81357 was used in western blot to study the interaction of Trf3 and Taf3 during early development and hematopoiesis in zebrafish
|Hart DO,Santra MK,Raha T,Green MR||Developmental dynamics : an official publication of the American Association of Anatomists (238:2540)||2009|
A Rictor-Myo1c complex participates in dynamic cortical actin events in 3T3-L1 adipocytes.
MA1-81357 was used in western blot to study the role of a Rictor-Myo1c complex in dynamic cortical actin events in 3T3-L1 adipocytes.
|Hagan GN,Lin Y,Magnuson MA,Avruch J,Czech MP||Molecular and cellular biology (28:4215)||2008|
DKC1 is a direct and conserved transcriptional target of c-MYC.
MA1-81357 was used in western blot to investigate the relationship between DKC1 and c-MYC
|Alawi F,Lee MN||Biochemical and biophysical research communications (362:893)||2007|
Cytoplasmic localized ubiquitin ligase cullin 7 binds to p53 and promotes cell growth by antagonizing p53 function.
MA1-81357 was used in western blot to study the association between ubiquitin ligase cullin 7 binds and p53
|Andrews P,He YJ,Xiong Y||Oncogene (25:4534)||2006|
N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.
MA1-81357 was used in western blot to investigate N-cadherin processing by proteolysis and the formation of N-cadherin-catenin complexes
|Wahl JK,Kim YJ,Cullen JM,Johnson KR,Wheelock MJ||The Journal of biological chemistry (278:17269)||2003|
14-3-3beta binds to and negatively regulates the tuberous sclerosis complex 2 (TSC2) tumor suppressor gene product, tuberin.
MA1-81357 was used in western blot to study the role of 14-3-3beta in regulating the TSC2 tumor suppressor gene product tuberin
|Shumway SD,Li Y,Xiong Y||The Journal of biological chemistry (278:2089)||2003|
Antibody library screens using detergent-solubilized mammalian cell lysates as antigen sources.
MA1-81357 was used in flow cytometry to perform high-throughput antibody screening using detergent-solubilized and biotinylated whole-cell lysates as the antigen source
|Cho YK,Shusta EV||Protein engineering, design and selection : PEDS (23:567)||2010|
|Not Applicable||Not Cited||
Unconventional myosin Myo1c promotes membrane fusion in a regulated exocytic pathway.
MA1-81357 was used in immunocytochemistry to study the role of Myo1c in the membrane fusion of GLUT4-containing vesicles with the adipocyte plasma membrane
|Bose A,Robida S,Furcinitti PS,Chawla A,Fogarty K,Corvera S,Czech MP||Molecular and cellular biology (24:5447)||2004|
EHD2 and the novel EH domain binding protein EHBP1 couple endocytosis to the actin cytoskeleton.
MA1-81357 was used in immunocytochemistry to study the role of EHD2 and EHBP1 in coupling endocytosis to the actin cytoskeleton
|Guilherme A,Soriano NA,Bose S,Holik J,Bose A,Pomerleau DP,Furcinitti P,Leszyk J,Corvera S,Czech MP||The Journal of biological chemistry (279:10593)||2004|
Cloning of mammalian Ire1 reveals diversity in the ER stress responses.
MA1-81357 was used in immunocytochemistry to demonstrate that Ire1 plays a role in multiple facets of the ER stress-response
|Wang XZ,Harding HP,Zhang Y,Jolicoeur EM,Kuroda M,Ron D||The EMBO journal (17:5708)||1998|
A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes.
MA1-81357 was used in immunocytochemistry to study how Rat7p regulates nucleocytoplasmic export of mRNA
|Gorsch LC,Dockendorff TC,Cole CN||The Journal of cell biology (129:939)||1995|