Western blot analysis of c-Myc was performed by loading 50 ug of NCCIT and Jurkat lysates, and 10 ug of Myc-MYCBP-containing cell lysate onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated c-Myc monoclonal antibody (Product # MA1-980-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075) at an exposure time of 2 min (NCCIT and Jurkat) and 3 sec (Myc-MYCBP). Results show a band at ~57kDa (NCCIT and Jurkat) and 16kDa (Myc-MYCBP) as well as a 2 unknown bands at 35-40kDa.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Synthetic peptide A(408) E E Q K L I S E E D L L R K R R E Q L K H K L E Q L R N S C A(438) of human c-Myc.|
|Storage buffer||proprietary buffer with proprietary stabilizer|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:250-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-980-HRP detects c-myc protein and c-myc tagged proteins.
MA1-980-HRP has been successfully used in Western blot procedures.
The MA1-980-HRP immunogen corresponds to the synthetic peptide A(408) E E Q K L I S E E D L L R K R R E Q L K H K L E Q L R N S C A(438) of human c-Myc.
The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation. In addition, deregulated expression of c-Myc induces apoptosis in different cell types, with c-myc requiring p53 for apoptosis in many cell types. This fact indicates heterogeneous mechanisms for c-myc-induced apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.