Invitrogen

c-Myc Monoclonal Antibody (9E10), HRP

Product Image Gallery

c-Myc Antibody in Western Blot (WB) — Published figure using c-Myc monoclonal antibody (Product # MA1-81357) in Western Blot

c-Myc Antibody in Immunoprecipitation (IP) — Published figure using c-Myc monoclonal antibody (Product # MA1-81357) in Immunoprecipitation

c-Myc Antibody in ELISA (ELISA) — Figure 1 Genomic integration of chimeric B cell receptors (CBCR) by CRISPR-Cas9-mediated HDR. (A) Schematic shows the design of the CBCR, which consists of an scFv-based antigen recognition domain (specific for a hen-egg lysozyme [HEL]) (orange), an extracellular spacer with a detection tag, a transmembrane (TM) domain (light blue), and endodomains (blue, purple). scFV, single chain variable fragment; VL, light chain variable domain; VH, heavy chain variable domain. (B) Shown is the gRNA sequence (blue) for Cas9-targeting of the safe-harbor locus Rosa26 locus; also shown is the corresponding PAM sequence (orange) and the beginning of the homology arms in the HDR template (light gray). The two blue arrows indicate the predicted Cas9 double-stranded break site. (C) CRISPR-Cas9-mediated HDR for genomic integration of CBCR construct into the Rosa26 locus. The PCR-linearized donor template contains a GFP reporter gene followed by a T2A coding sequence, the Vkappa signal peptide, the CBCR cassette and a poly-A sequence all under the control of a CMV promoter and flanked by sequences homologous to the Rosa26 locus next to the gRNA target site of 1.4 and 1.5 kb, respectively (light purple). T2A: the self-cleaving thosea asigna virus 2A sequence, pA: SV40 polyA sequence. (D) Schematic shows the format of the CBCR antigen binding domain including a linker with a detection tag, either Myc or Flag epitope. (E) Cells were enriched for GFP (488 nm) expression via FACS. Graph shows ELISA re

Product Details

MA1-81357

Applications	Tested Dilution	Publications
Western Blot (WB)	1/100 - 1/500	View 13 publications 13 publications
Immunohistochemistry (Paraffin) (IHC (P))	Assay-dependent	-
Immunohistochemistry (Frozen) (IHC (F))	Assay-dependent	-
ELISA (ELISA)	1/100 - 1/500	View 1 publication 1 publication
Immunoprecipitation (IP)	-	View 5 publications 5 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunocytochemistry (ICC/IF)	-	View 4 publications 4 publications

Product Specifications
Species Reactivity	Human
Published species	Bovine, Human, Invertebrate, Mouse, Yeast
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	9E10
Immunogen	Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of human c-myc
Conjugate	HRP
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with proprietary stabilizer
Contains	0.01% thimerosal
Storage conditions	4° C, do not freeze
RRID	AB_930031

Product Specific Information

This product does not require protein digestion or antigen retrieval prior to staining of paraffin sections. Non-reducing conditions are recommended for Western blot applications.

Mouse anti c-myc antibody, clone 9E10 detects the p62c-myc proto-oncogene protein, which is involved in the regulation of the cell cycle and cell growth.

Target Information

The c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies have shown that c-Myc is essential for vasculogenesis and angiogenesis in tumor development, which distributes blood throughout the cells. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation, and the deregulated expression of c-Myc induces apoptosis in different cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The SUMOylation of TAB2 mediated by TRIM60 inhibits MAPK/NF-κB activation and the innate immune response.

Cellular & molecular immunology

Gu Z,Chen X,Yang W,Qi Y,Yu H,Wang X,Gong Y,Chen Q,Zhong B,Dai L,Qi S,Zhang Z,Zhang H,Hu H

MA1-81357 was used in Western Blot to reveal that SUMOylation of TAB2 mediated by TRIM60 is a novel mechanism for regulating the innate immune response, potentially paving the way for a new strategy to control antibacterial immune responses.

Sun Aug 01 00:00:00 EDT 2021

Molecular Design, Optimization, and Genomic Integration of Chimeric B Cell Receptors in Murine B Cells.

Frontiers in immunology

Pesch T,Bonati L,Kelton W,Parola C,Ehling RA,Csepregi L,Kitamura D,Reddy ST

Published figure using c-Myc monoclonal antibody (Product # MA1-81357) in ELISA

Tue Nov 10 00:00:00 EST 2020

AvrRpm1 Functions as an ADP-Ribosyl Transferase to Modify NOI Domain-Containing Proteins, Including Arabidopsis and Soybean RPM1-Interacting Protein4.

The Plant cell

Redditt TJ,Chung EH,Karimi HZ,Rodibaugh N,Zhang Y,Trinidad JC,Kim JH,Zhou Q,Shen M,Dangl JL,Mackey D,Innes RW

Published figure using c-Myc monoclonal antibody (Product # MA1-81357) in Immunoprecipitation

Fri Nov 01 00:00:00 EDT 2019

AvrRpm1 Functions as an ADP-Ribosyl Transferase to Modify NOI Domain-Containing Proteins, Including Arabidopsis and Soybean RPM1-Interacting Protein4.

The Plant cell

Redditt TJ,Chung EH,Karimi HZ,Rodibaugh N,Zhang Y,Trinidad JC,Kim JH,Zhou Q,Shen M,Dangl JL,Mackey D,Innes RW

Published figure using c-Myc monoclonal antibody (Product # MA1-81357) in Immunoprecipitation

Fri Nov 01 00:00:00 EDT 2019

Identification of two novel Shank3 transcripts in the developing mouse neocortex.

Journal of neurochemistry

Waga C,Asano H,Sanagi T,Suzuki E,Nakamura Y,Tsuchiya A,Itoh M,Goto Y,Kohsaka S,Uchino S

MA1-81357 was used in immunohistochemistry to study the role in the developing murine neocortex of two novel transcripts of the Shank3 synaptic scaffold protein

Wed Jan 01 00:00:00 EST 2014

MAPK Hog1 closes the S. cerevisiae glycerol channel Fps1 by phosphorylating and displacing its positive regulators.

Genes & development

Lee J,Reiter W,Dohnal I,Gregori C,Beese-Sims S,Kuchler K,Ammerer G,Levin DE

MA1-81357 was used in immunoprecipitation and western blot to study the regulation of the Saccharomyces cerevisiae Fps1 glycerol channel by Hog1-mediated phosphorylation

Sun Dec 01 00:00:00 EST 2013

Fulvestrant induces resistance by modulating GPER and CDK6 expression: implication of methyltransferases, deacetylases and the hSWI/SNF chromatin remodelling complex.

British journal of cancer

Giessrigl B,Schmidt WM,Kalipciyan M,Jeitler M,Bilban M,Gollinger M,Krieger S,Jäger W,Mader RM,Krupitza G

MA1-81357 was used in western blot to study the roles of GPER and CDK6 in the mechanisms of fulvestrant resistance in ER-positive breast cancer cells

Tue Nov 12 00:00:00 EST 2013

Functional impact of Aurora A-mediated phosphorylation of HP1γ at serine 83 during cell cycle progression.

Epigenetics & chromatin

Grzenda A,Leonard P,Seo S,Mathison AJ,Urrutia G,Calvo E,Iovanna J,Urrutia R,Lomberk G

MA1-81357 was used in western blot to study the cell cycle role of HP1-gamma phosphorylation at Ser 83 by Aurora A

Fri Jul 05 00:00:00 EDT 2013

Activation of a PGC-1-related coactivator (PRC)-dependent inflammatory stress program linked to apoptosis and premature senescence.

The Journal of biological chemistry

Gleyzer N,Scarpulla RC

MA1-81357 was used in western blot to study the activation of the PRC-dependent stress program by apoptosis and senescence and its role in the response to cellular dysfunction

Fri Mar 22 00:00:00 EDT 2013

The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A.

Biology open

Moghe S,Jiang F,Miura Y,Cerny RL,Tsai MY,Furukawa M

MA1-81357 was used in western blot to study the role of Aurora A kinase in the mechanism by which CUL3-KLHL18 regulates cell cycle entry

Wed Feb 15 00:00:00 EST 2012

Regulation of fertility, survival, and cuticle collagen function by the Caenorhabditis elegans eaf-1 and ell-1 genes.

The Journal of biological chemistry

Cai L,Phong BL,Fisher AL,Wang Z

MA1-81357 was used in western blot to study the in vivo functions of the EAF2 and ELL family proteins in Caenorhabditis elegans

Fri Oct 14 00:00:00 EDT 2011

Differential expression of MYC in H. pylori-related intestinal and diffuse gastric tumors.

Virchows Archiv : an international journal of pathology

Silva-Fernandes IJ,Alves MK,Lima VP,de Lima MA,Barros MA,Ferreira MV,Rabenhorst SH

MA1-81357 was used in immunohistochemistry to characterize the expression of cell cycle proteins in Helicobacter-associated gastric cancer

Wed Jun 01 00:00:00 EDT 2011

Selective interaction between Trf3 and Taf3 required for early development and hematopoiesis.

Developmental dynamics : an official publication of the American Association of Anatomists

Hart DO,Santra MK,Raha T,Green MR

MA1-81357 was used in western blot to study the interaction of Trf3 and Taf3 during early development and hematopoiesis in zebrafish

Thu Oct 01 00:00:00 EDT 2009

A Rictor-Myo1c complex participates in dynamic cortical actin events in 3T3-L1 adipocytes.

Molecular and cellular biology

Hagan GN,Lin Y,Magnuson MA,Avruch J,Czech MP

MA1-81357 was used in western blot to study the role of a Rictor-Myo1c complex in dynamic cortical actin events in 3T3-L1 adipocytes.

Tue Jul 01 00:00:00 EDT 2008

Relationship between EBV infection and expression of cellular proteins c-Myc, Bcl-2, and Bax in gastric carcinomas.

Diagnostic molecular pathology : the American journal of surgical pathology, part B

Lima MA,Ferreira MV,Barros MA,Pardini MI,Ferrasi AC,Mota RM,Rabenhorst SH

MA1-81357 was used in immunohistochemistry to study the relationship between EBV infection and expression of c-Myc, Bcl-2, and Bax in gastric carcinomas

Sun Jun 01 00:00:00 EDT 2008

WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase.

Genes & development

Hu J,Zacharek S,He YJ,Lee H,Shumway S,Duronio RJ,Xiong Y

MA1-81357 was used in immunoprecipitation to study the role of the WD40 protein FBW5 in promoting the ubiquitinylation of tumor suppresor protein TSC2

Tue Apr 01 00:00:00 EDT 2008

DKC1 is a direct and conserved transcriptional target of c-MYC.

Biochemical and biophysical research communications

Alawi F,Lee MN

MA1-81357 was used in western blot to investigate the relationship between DKC1 and c-MYC

Sat Nov 03 00:00:00 EDT 2007

Cytoplasmic localized ubiquitin ligase cullin 7 binds to p53 and promotes cell growth by antagonizing p53 function.

Oncogene

Andrews P,He YJ,Xiong Y

MA1-81357 was used in western blot to study the association between ubiquitin ligase cullin 7 binds and p53

Thu Aug 03 00:00:00 EDT 2006

Unconventional myosin Myo1c promotes membrane fusion in a regulated exocytic pathway.

Molecular and cellular biology

Bose A,Robida S,Furcinitti PS,Chawla A,Fogarty K,Corvera S,Czech MP

MA1-81357 was used in immunocytochemistry to study the role of Myo1c in the membrane fusion of GLUT4-containing vesicles with the adipocyte plasma membrane

Tue Jun 01 00:00:00 EDT 2004

EHD2 and the novel EH domain binding protein EHBP1 couple endocytosis to the actin cytoskeleton.

The Journal of biological chemistry

Guilherme A,Soriano NA,Bose S,Holik J,Bose A,Pomerleau DP,Furcinitti P,Leszyk J,Corvera S,Czech MP

MA1-81357 was used in immunocytochemistry to study the role of EHD2 and EHBP1 in coupling endocytosis to the actin cytoskeleton

Fri Mar 12 00:00:00 EST 2004

N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.

The Journal of biological chemistry

Wahl JK,Kim YJ,Cullen JM,Johnson KR,Wheelock MJ

MA1-81357 was used in western blot to investigate N-cadherin processing by proteolysis and the formation of N-cadherin-catenin complexes

Fri May 09 00:00:00 EDT 2003

14-3-3beta binds to and negatively regulates the tuberous sclerosis complex 2 (TSC2) tumor suppressor gene product, tuberin.

The Journal of biological chemistry

Shumway SD,Li Y,Xiong Y

MA1-81357 was used in western blot to study the role of 14-3-3beta in regulating the TSC2 tumor suppressor gene product tuberin

Fri Jan 24 00:00:00 EST 2003

ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity.

Molecular cell

Ohta T,Michel JJ,Schottelius AJ,Xiong Y

MA1-81357 was used in immunoprecipitation to investigate the interaction between ROC1 and cullin proteins

Thu Apr 01 00:00:00 EST 1999

Cloning of mammalian Ire1 reveals diversity in the ER stress responses.

The EMBO journal

Wang XZ,Harding HP,Zhang Y,Jolicoeur EM,Kuroda M,Ron D

MA1-81357 was used in immunocytochemistry to demonstrate that Ire1 plays a role in multiple facets of the ER stress-response

Thu Oct 01 00:00:00 EDT 1998

A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes.

The Journal of cell biology

Gorsch LC,Dockendorff TC,Cole CN

MA1-81357 was used in immunocytochemistry to study how Rat7p regulates nucleocytoplasmic export of mRNA

Mon May 01 00:00:00 EDT 1995

SNAP receptors implicated in vesicle targeting and fusion.

Nature

Söllner T,Whiteheart SW,Brunner M,Erdjument-Bromage H,Geromanos S,Tempst P,Rothman JE

MA1-81357 was used in immunoprecipitation to characterize SNAP receptors isolated from bovine brain

Thu Mar 25 00:00:00 EST 1993

Bioinformatics

Protein Aliases: avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; c-myc epitope tag; Class E basic helix-loop-helix protein 39; cmyc; Myc proto-oncogene protein; myc-related translation/localization regulatory factor; Proto-oncogene c-Myc; Transcription factor p64; v-myc myelocytomatosis viral oncogene homolog

Gene Aliases: BHLHE39; c-Myc; MRTL; MYC; MYCC

UniProt ID: (Human) P01106

Entrez Gene ID: (Human) 4609

Molecular Function: basic helix-loop-helix transcription factor

Function(s)

negative regulation of transcription from RNA polymerase II promoter MAPK cascade branching involved in ureteric bud morphogenesis positive regulation of mesenchymal cell proliferation energy reserve metabolic process chromatin remodeling transcription from RNA polymerase II promoter cellular iron ion homeostasis cellular response to DNA damage stimulus cell cycle arrest Notch signaling pathway positive regulation of cell proliferation response to gamma radiation regulation of gene expression oxygen transport regulation of telomere maintenance negative regulation of stress-activated MAPK cascade cellular response to UV cellular response to drug response to drug negative regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process fibroblast apoptotic process negative regulation of monocyte differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation negative regulation of fibroblast proliferation positive regulation of epithelial cell proliferation chromosome organization negative regulation of cell division canonical Wnt signaling pathway response to growth factor positive regulation of metanephric cap mesenchymal cell proliferation beta-catenin-TCF complex assembly positive regulation of DNA biosynthetic process positive regulation of response to DNA damage stimulus

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein complex binding protein dimerization activity repressing transcription factor binding E-box binding

c-Myc Monoclonal Antibody (9E10), HRP

Product Details

MA1-81357

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

ELISA (ELISA)

Immunoprecipitation (IP)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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