|Tested species reactivity||Tag|
|Published species reactivity||Rabbit, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C E(410) Q K L I S E E D L(419) of human c-Myc.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-981 detects c-Myc tagged proteins.
PA1-981 has been successfully used in WB and immunofluorescence procedures to detect the presence of expressed c-Myc tagged proteins in transfected mammalian cells.
PA1-981 immunogen is a synthetic peptide corresponding to residues C E(410) Q K L I S E E D L(419) of human c-Myc. PA1-981 immunizing peptide (PEP-035) is available for use in neutralization and control experiments.
The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation. In addition, deregulated expression of c-Myc induces apoptosis in different cell types, with c-myc requiring p53 for apoptosis in many cell types. This fact indicates heterogeneous mechanisms for c-myc-induced apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Excessive sarcoplasmic/endoplasmic reticulum Ca2+-ATPase expression causes increased sarcoplasmic reticulum Ca2+ uptake but decreases myocyte shortening.
PA1-981 was used in western blot to study the effect of sarcoplasmic/endoplasmic reticulum calcium-ATPase expression on sarcoplasmic reticulum calcium uptake and myocyte contractility
|Teucher N,Prestle J,Seidler T,Currie S,Elliott EB,Reynolds DF,Schott P,Wagner S,Kogler H,Inesi G,Bers DM,Hasenfuss G,Smith GL||Circulation (110:3553)||2004|
Increased expression of the glial glutamate transporter EAAT2 modulates excitotoxicity and delays the onset but not the outcome of ALS in mice.
PA1-981 was used in immunohistochemistry to study the role of EAAT2 in the degeneration of motor neuron in ALS.
|Guo H,Lai L,Butchbach ME,Stockinger MP,Shan X,Bishop GA,Lin CL||Human molecular genetics (12:2519)||2003|