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Immunofluorescence analysis of c-Myc was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with c-Myc (9 E11) Mouse Monoclonal Antibody (AHO0052) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Mouse , Chicken , Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||A synthetic peptide, corresponding to amino acid residues 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from the C terminus of c-myc,coupled to KLH.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg x 10^6 cells|
|Immunoprecipitation (IP)||10 ul|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
c-Myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Nird, c-myc, AU016757, bHLHe39, MYCC, Niard, MRTL, Myc2, c-Myc
c-myc proto-oncogene, myc proto-oncogene protein, transcription factor p64, avian myelocytomatosis viral oncogene homolog, class E basic helix-loop-helix protein 39, myc-related translation/localization regulatory factor, proto-oncogene c-Myc, v-myc myelocytomatosis viral oncogene homolog, c Myc, Cellular myelocytomatosis oncogene, Myc protein, Myc proto oncogene protein, Myc2, Niard, Nird, v myc avian myelocytomatosis viral oncogene hom