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|Tested species reactivity||Tag|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||c-Myc synthetic peptide: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||1:1000-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
NOTE: R950-25 was previously sold under product #46-0603. When re-ordering, please use #R950-25.
This antibody recognizes amino acid sequence: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-.
This product contains enough material for 25 Western blots. Western blot against 500ng of recombinant protein gave a strong signal using chemiluminescent or alkaline phosphatase detection reagents. For Western blot, dilute in PBS containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM).
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The regulation of autophagosome dynamics by huntingtin and HAP1 is disrupted by expression of mutant huntingtin, leading to defective cargo degradation.
R950-25 was used in western blot to investigate the role of huntingtin in the the regulation of autophagosome dynamics.
|Wong YC,Holzbaur EL||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:1293)||2014|
46-0603; Myc; Niard; Nird; 460603; avian myelocytomatosis viral oncogene homolog; bHLHe39; Cellular myelocytomatosis oncogene; Class E basic helix-loop-helix protein 39; Myc proto oncogene protein; Myc proto-oncogene protein; myc-related translation/localization regulatory factor; Myc2; Proto-oncogene c-Myc; R950-25; R95025; Transcription factor p64; v myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog
BHLHE39; c-Myc; MRTL; MYC; MYCC