|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Western Blot (WB)||1:1000-1:5000|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Tag|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||c-Myc synthetic peptide: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-|
|Contains||0.01% sodium azide|
|Storage conditions||4° C, store in dark|
R953-25 recognizes amino acid sequence: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-.
This antibody has been tested in IF experiments using cultured CHO (Chinese Hamster ovary) cells expressing recombinant epitope-tagged fusion proteins. For IF, dilute the FITC-conjugated antibody in PBS containing 10% fetal bovine serum (FBS). The amount of antibody provided is sufficient for 25 immunostaining reactions using a 1 mL working solution per reaction.
This antibody has also been tested against purified Positope™ control protein. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His(C-term), HisG, c-myc, and V5.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: avian myelocytomatosis viral oncogene homolog; bHLHe39; Cellular myelocytomatosis oncogene; Class E basic helix-loop-helix protein 39; Myc; Myc proto oncogene protein; Myc proto-oncogene protein; myc-related translation/localization regulatory factor; Myc2; Niard; Nird; Proto-oncogene c-Myc; Transcription factor p64; v myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog
Gene Aliases: BHLHE39; c-Myc; MRTL; MYC; MYCC
UniProt ID: (Human) P01106
Entrez Gene ID: (Human) 4609
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.