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Immunofluorescent analysis of c-Myc (green) in H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a c-Myc monoclonal antibody (Product # MA1-980) at a dilution of 1:200 overnight at 4°C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Yeast, Rat, Mouse, Human, Xenopus|
|Host / Isotype||Mouse / IgG|
|Immunogen||Synthetic peptide A(408) E E Q K L I S E E D L L R K R R E Q L K H K L E Q L R N S C A(438) of human c-Myc.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay Dependent|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||1:50-1:200|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:1000|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-980 detects c-myc protein and c-myc tagged proteins.
MA1-980 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunofluorescence, ELISA, flow cytometry, and immunoprecipitation procedures.
The MA1-980 immunogen corresponds to the synthetic peptide A(408) E E Q K L I S E E D L L R K R R E Q L K H K L E Q L R N S C A(438) of human c-Myc.
The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation. In addition, deregulated expression of c-Myc induces apoptosis in different cell types, with c-myc requiring p53 for apoptosis in many cell types. This fact indicates heterogeneous mechanisms for c-myc-induced apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Identification of two novel Shank3 transcripts in the developing mouse neocortex.
MA1-980 was used in immunohistochemistry to study the role in the developing murine neocortex of two novel transcripts of the Shank3 synaptic scaffold protein
|Waga C,Asano H,Sanagi T,Suzuki E,Nakamura Y,Tsuchiya A,Itoh M,Goto Y,Kohsaka S,Uchino S||Journal of neurochemistry (128:280)||2014|
Relationship between EBV infection and expression of cellular proteins c-Myc, Bcl-2, and Bax in gastric carcinomas.
MA1-980 was used in immunohistochemistry to study the relationship between EBV infection and expression of c-Myc, Bcl-2, and Bax in gastric carcinomas
|Lima MA,Ferreira MV,Barros MA,Pardini MI,Ferrasi AC,Mota RM,Rabenhorst SH||Diagnostic molecular pathology : the American journal of surgical pathology, part B (17:82)||2008|
MAPK Hog1 closes the S. cerevisiae glycerol channel Fps1 by phosphorylating and displacing its positive regulators.
MA1-980 was used in immunoprecipitation and western blot to study the regulation of the Saccharomyces cerevisiae Fps1 glycerol channel by Hog1-mediated phosphorylation
|Lee J,Reiter W,Dohnal I,Gregori C,Beese-Sims S,Kuchler K,Ammerer G,Levin DE||Genes & development (27:2590)||2013|
Detection of protein-RNA complexes in Xenopus oocytes.
MA1-980 was used in immunoprecipitation to develop a method for detecting protein-RNA complexes in Xenopus oocytes
|Huber PW,Zhao WM||Methods (San Diego, Calif.) (51:82)||2010|
WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase.
MA1-980 was used in immunoprecipitation to study the role of the WD40 protein FBW5 in promoting the ubiquitinylation of tumor suppresor protein TSC2
|Hu J,Zacharek S,He YJ,Lee H,Shumway S,Duronio RJ,Xiong Y||Genes & development (22:866)||2008|
ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity.
MA1-980 was used in immunoprecipitation to investigate the interaction between ROC1 and cullin proteins
|Ohta T,Michel JJ,Schottelius AJ,Xiong Y||Molecular cell (3:535)||1999|
Regulation of the transcriptional activation of the androgen receptor by the UXT-binding protein VHL.
MA1-980 was used in immunoprecipitation and western blot to study the mechanism by which the UXT-binding protein VHL modulates androgen receptor transcriptional activation
|Chen S,Chen K,Zhang Q,Cheng H,Zhou R||The Biochemical journal (456:55)||2013|
Fulvestrant induces resistance by modulating GPER and CDK6 expression: implication of methyltransferases, deacetylases and the hSWI/SNF chromatin remodelling complex.
MA1-980 was used in western blot to study the roles of GPER and CDK6 in the mechanisms of fulvestrant resistance in ER-positive breast cancer cells
|Giessrigl B,Schmidt WM,Kalipciyan M,Jeitler M,Bilban M,Gollinger M,Krieger S,Jäger W,Mader RM,Krupitza G||British journal of cancer (109:2751)||2013|
The SUMOylation of zinc-fingers and homeoboxes 1 (ZHX1) by Ubc9 regulates its stability and transcriptional repression activity.
MA1-980 was used in western blot to study the mechanism by which Ubc9-mediated SUMOylation of ZHX1 modulates its stability and functional activity
|Chen S,Yu X,Lei Q,Ma L,Guo D||Journal of cellular biochemistry (114:2323)||2013|
SIRT1 collaborates with ATM and HDAC1 to maintain genomic stability in neurons.
MA1-980 was used in western blot to study the roles of SIRT1, ATM and HDAC1 in the DNA double-strand break response of neurons and the therapeutic significance for neurodegenerative disease
|Dobbin MM,Madabhushi R,Pan L,Chen Y,Kim D,Gao J,Ahanonu B,Pao PC,Qiu Y,Zhao Y,Tsai LH||Nature neuroscience (16:1008)||2013|
Activation of a PGC-1-related coactivator (PRC)-dependent inflammatory stress program linked to apoptosis and premature senescence.
MA1-980 was used in western blot to study the activation of the PRC-dependent stress program by apoptosis and senescence and its role in the response to cellular dysfunction
|Gleyzer N,Scarpulla RC||The Journal of biological chemistry (288:8004)||2013|
Selective interaction between Trf3 and Taf3 required for early development and hematopoiesis.
MA1-980 was used in western blot to study the interaction of Trf3 and Taf3 during early development and hematopoiesis in zebrafish
|Hart DO,Santra MK,Raha T,Green MR||Developmental dynamics : an official publication of the American Association of Anatomists (238:2540)||2009|
Akt phosphorylates both Tsc1 and Tsc2 in Drosophila, but neither phosphorylation is required for normal animal growth.
MA1-980 was used in western blot to investigate the phosphorylation of Tsc1 and Tsc2
|Schleich S,Teleman AA||PloS one (4:null)||2009|
Limited functional and metabolic improvements in hypertrophic and healthy rat heart overexpressing the skeletal muscle isoform of SERCA1 by adenoviral gene transfer in vivo.
MA1-980 was used in western blot to study the results of adenoviral SERCA1 gene transfer in Sprague-Dawley rat hearts.
|O'Donnell JM,Fields A,Xu X,Chowdhury SA,Geenen DL,Bi J||American journal of physiology. Heart and circulatory physiology (295:H2483)||2008|
A Rictor-Myo1c complex participates in dynamic cortical actin events in 3T3-L1 adipocytes.
MA1-980 was used in western blot to study the role of a Rictor-Myo1c complex in dynamic cortical actin events in 3T3-L1 adipocytes.
|Hagan GN,Lin Y,Magnuson MA,Avruch J,Czech MP||Molecular and cellular biology (28:4215)||2008|
DKC1 is a direct and conserved transcriptional target of c-MYC.
MA1-980 was used in western blot to investigate the relationship between DKC1 and c-MYC
|Alawi F,Lee MN||Biochemical and biophysical research communications (362:893)||2007|
Cytoplasmic localized ubiquitin ligase cullin 7 binds to p53 and promotes cell growth by antagonizing p53 function.
MA1-980 was used in western blot to study the association between ubiquitin ligase cullin 7 binds and p53
|Andrews P,He YJ,Xiong Y||Oncogene (25:4534)||2006|
N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.
MA1-980 was used in western blot to investigate N-cadherin processing by proteolysis and the formation of N-cadherin-catenin complexes
|Wahl JK,Kim YJ,Cullen JM,Johnson KR,Wheelock MJ||The Journal of biological chemistry (278:17269)||2003|
14-3-3beta binds to and negatively regulates the tuberous sclerosis complex 2 (TSC2) tumor suppressor gene product, tuberin.
MA1-980 was used in western blot to study the role of 14-3-3beta in regulating the TSC2 tumor suppressor gene product tuberin
|Shumway SD,Li Y,Xiong Y||The Journal of biological chemistry (278:2089)||2003|
Functional analyses of melanocortin-4 receptor mutations identified from patients with binge eating disorder and nonobese or obese subjects.
MA1-980 was used in immunocytochemistry to study the role of MC4R mutations in the pathogenesis of binge eating disorder.
|Tao YX,Segaloff DL||The Journal of clinical endocrinology and metabolism (90:5632)||2005|
EHD2 and the novel EH domain binding protein EHBP1 couple endocytosis to the actin cytoskeleton.
MA1-980 was used in immunocytochemistry to study the role of EHD2 and EHBP1 in coupling endocytosis to the actin cytoskeleton
|Guilherme A,Soriano NA,Bose S,Holik J,Bose A,Pomerleau DP,Furcinitti P,Leszyk J,Corvera S,Czech MP||The Journal of biological chemistry (279:10593)||2004|
Deletion of codons 88-92 of the melanocortin-4 receptor gene: a novel deleterious mutation in an obese female.
MA1-980 was used in immunocytochemistry to investigate the role of the melanocortin-4 receptor in obesity.
|Donohoue PA,Tao YX,Collins M,Yeo GS,O'Rahilly S,Segaloff DL||The Journal of clinical endocrinology and metabolism (88:5841)||2003|
Cloning of mammalian Ire1 reveals diversity in the ER stress responses.
MA1-980 was used in immunocytochemistry to demonstrate that Ire1 plays a role in multiple facets of the ER stress-response
|Wang XZ,Harding HP,Zhang Y,Jolicoeur EM,Kuroda M,Ron D||The EMBO journal (17:5708)||1998|
A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes.
MA1-980 was used in immunocytochemistry to study how Rat7p regulates nucleocytoplasmic export of mRNA
|Gorsch LC,Dockendorff TC,Cole CN||The Journal of cell biology (129:939)||1995|
avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; Cellular myelocytomatosis oncogene; Class E basic helix-loop-helix protein 39; Myc proto oncogene protein; Myc proto-oncogene protein; myc-related translation/localization regulatory factor; Myc2; Niard; Nird; Proto-oncogene c-Myc; Transcription factor p64; v myc avian myelocytomatosis viral oncogene hom; v-myc myelocytomatosis viral oncogene homolog
BHLHE39; c-Myc; MRTL; MYC; MYCC