|ELISA (ELISA)||Assay dependent|
|Immunofluorescence (IF)||1:500 - 1:2000|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000 - 1:3000|
|Western Blot (WB)||See 5 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Tag|
|Published species reactivity||Plant , Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide: EQKLISEEDL (Myc).|
|Storage buffer||PBS, pH 7.2|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-21316 detects N-terminal or C-terminal Myc tagged fusion proteins.
MA1-21316 has been successfully used in ELISA, immunofluorescence, immunoprecipitation and Western Blot procedures.
MA1-21316 immunogen is a synthetic peptide EQKLISEEDL (Myc).
MA1-21316 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: avian myelocytomatosis viral oncogene homolog; bHLHe39; Class E basic helix-loop-helix protein 39; MRTL; Myc proto-oncogene protein; myc-related translation/localization regulatory factor; Proto-oncogene c-Myc; Transcription factor p64; v-myc myelocytomatosis viral oncogene homolog
Gene Aliases: BHLHE39; c-Myc; MRTL; MYC; MYCC
UniProt ID: (Human) P01106
Entrez Gene ID: (Human) 4609
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.