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|Tested species reactivity||Tag|
|Published species reactivity||Plant, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide: EQKLISEEDL (Myc).|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunofluorescence (IF)||1:500 - 1:2000|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000 - 1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
MA1-21316 detects N-terminal or C-terminal Myc tagged fusion proteins.
MA1-21316 has been successfully used in ELISA, immunofluorescence, immunoprecipitation and Western Blot procedures.
MA1-21316 immunogen is a synthetic peptide EQKLISEEDL (Myc).
MA1-21316 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AP1S3 mutations are associated with pustular psoriasis and impaired Toll-like receptor 3 trafficking.
MA1-21316 was used in western blot to study the role of TLR3 vesicular trafficking defects in the mechanism by which AP1S3 mutations promote pustular psoriasis
|Setta-Kaffetzi N,Simpson MA,Navarini AA,Patel VM,Lu HC,Allen MH,Duckworth M,Bachelez H,Burden AD,Choon SE,Griffiths CE,Kirby B,Kolios A,Seyger MM,Prins C,Smahi A,Trembath RC,Fraternali F,Smith CH,Barker JN,Capon F||American journal of human genetics (94:790)||2014|
Xyloglucan xylosyltransferases XXT1, XXT2, and XXT5 and the glucan synthase CSLC4 form Golgi-localized multiprotein complexes.
MA1-21316 was used in western blot to study the protein composition of multiprotein complexes involved in xyloglucan biosynthesis
|Chou YH,Pogorelko G,Zabotina OA||Plant physiology (159:1355)||2012|
avian myelocytomatosis viral oncogene homolog; bHLHe39; Class E basic helix-loop-helix protein 39; MRTL; Myc proto-oncogene protein; myc-related translation/localization regulatory factor; Proto-oncogene c-Myc; Transcription factor p64; v-myc myelocytomatosis viral oncogene homolog
BHLHE39; c-Myc; MRTL; MYC; MYCC