|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2a, kappa|
|Storage buffer||PBS, pH 7.2|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 5 publications below|
Description: The 1RELAH5 monoclonal antibody reacts with mouse c-Rel. c-Rel is a 65 kDa hematopoietic lineage-restricted member of the NF-kB family of transcription factors. In the thymus, c-Rel is upregulated in regulatory T cells (Treg) when compared to other CD4+ thymocytes. c-Rel is believed to play a critical role in thymic Treg (nTreg) development, but is not necessary for TGF beta-mediated T reg (iTreg) differentiation. C-Rel is also involved in proliferation and survival in mature T cells.
Applications Reported: This 1RELAH5 antibody has been reported for use in western blotting.
Applications Tested: This 1RELAH5 antibody has been tested by western blot analysis of mouse splenocytes. This can be used at 1-5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA, RELB (604758), NFKB1, and NFKB2. These proteins are related through a highly conserved N-terminal region termed the 'Rel domain,' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (Belguise and Sonenshein, 2007).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Foxp3 interacts with c-Rel to mediate NF-¿B repression.||Loizou L,Andersen KG,Betz AG||PloS one (6:null)||2011|
|Not Applicable||Not Cited||c-Rel is required for the development of thymic Foxp3+ CD4 regulatory T cells.||Isomura I,Palmer S,Grumont RJ,Bunting K,Hoyne G,Wilkinson N,Banerjee A,Proietto A,Gugasyan R,Wu L,Li W,McNally A,Steptoe RJ,Thomas R,Shannon MF,Gerondakis S||The Journal of experimental medicine (206:3001)||2009|
|Not Applicable||Not Cited||Development of Foxp3(+) regulatory t cells is driven by the c-Rel enhanceosome.||Ruan Q,Kameswaran V,Tone Y,Li L,Liou HC,Greene MI,Tone M,Chen YH||Immunity (31:932)||2009|
|Not Applicable||Not Cited||NF-kappaB regulation in the immune system.||Li Q,Verma IM||Nature reviews. Immunology (2:725)||2002|
|Not Applicable||Not Cited||Developmental expression of the mouse c-rel proto-oncogene in hematopoietic organs.||Carrasco D,Weih F,Bravo R||Development (Cambridge, England) (120:2991)||1994|