|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from internal region of human c-erbB3 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is colon carcinoma or breast carcinoma.
The gene for c-erbB-3/HER-3 is located on the long arm of human chromosome 12 (12q13) and is transcribed into a 6.2kb mRNA which is translated into a 160/180kDa glycoprotein. The c-erbB-3/HER-3 gene encodes secreted as well as transmembrane receptor tyrosine kinase due to alternative splicing. The c-erbB-3 is overexpressed in a variety of tumors including breast, stomach, pancreas, and colon
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.