Immunofluorescent analysis of cMAF (green) in RPMI 8226 and Daudi cells. Cells were immersion fixed in 4% PFA and permeabilized in 0.5% Triton X-100. Cells were stained with cMAF polyclonal antibody (Product # PA5-23179) at a dilution of 1:500 overnight at 4 degrees, washed in PBS followed by incubation with an Alexa 488 donkey anti-rabbit secondary antibody at a dilution of 1:100 for one hour at room temperature. Data courtesy of the Antibody Data Exchange Program.
|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (75 APSPGSGSEQKAHLED 90) of c-maf protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
c-maf is the cellular counterpart of oncogenic v-maf that belongs to the family of basic region leucine zipper domain transcription factors. The leucine-zipper domain is involved in the interaction with LRPICD. There are two forms of human c-maf mRNA, c-maf-long and c-maf-short. It is identified in the genome of the acute transforming avian retrovirus AS42. c-maf targets are IL-4 in Th2 cells, the crystalline genes in lens fiber cells, insulin gene in islet , p53 and L7 where it exerts its transcriptional role through binding to a Maf recognition element (MARE). It regulates Th2 differentiation and lineage-specific hematopoiesis. c-maf is a transcription factor for IL-10 gene expression in LPS-activated macrophages. Chromosomal aberration involving maf is found in some forms of multiple myeloma. It is expressed in myeloma cell lines and resting monocytes/macrophages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.