|Tested species reactivity||Human|
|Published species reactivity||Yeast, Zebrafish, Human, Mouse, Xenopus, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||A synthetic peptide, aa 408-439, (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:50|
|Immunomicroscopy (IM)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12077 targets c-myc in FACS, IF, IHC (P), IM, IP, and WB applications and shows reactivity with Human samples.
The MA5-12077 immunogen is a synthetic peptide, aa 408-439, (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc.
c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
BCL2, BCL6, IGH, TP53, and MYC protein expression and gene rearrangements as prognostic markers in diffuse large B-cell lymphoma: a study of 44 Turkish patients.
MA5-12077 was used in immunohistochemistry to study the prognostic value of immunohistochemical and FISH analysis of BCL2, BCL6, IGH, TP53, and MYC in Turkish patients with diffuse large B-cell lymphoma
|Akay OM,Aras BD,Isiksoy S,Toprak C,Mutlu FS,Artan S,Oner U,Gulbas Z||Cancer genetics (207:87)||2014|
EGFR and MYC gene copy number aberrations are more common in squamous cell carcinoma than keratoacanthoma: a FISH study.
MA5-12077 was used in immunohistochemistry to use FISH to study the higher frequency of EGFR and MYC gene amplification in squamous cell carcinoma than in keratoacanthoma
|Jacobs MS,Persons DL,Fraga GR||Journal of cutaneous pathology (40:447)||2013|
Expression of p53, Ki-67 and c-Myc proteins is predictive of the surgical molecular margin in colorectal carcinoma.
MA5-12077 was used in immunohistochemistry to study the value of p53, Ki-67 and c-myc expression in determining the surgical margin in colorectal carcinoma resection
|Li N,Wang J,Shen S,Bu X,Tian X,Huang P||Pathology oncology research : POR (17:479)||2011|
Delaying Gal4-driven gene expression in the zebrafish with morpholinos and Gal80.
MA5-12077 was used in immunohistochemistry and western blot to study the effect of morpholinos and Gal80 on gene expression driven by Gal40 in zebrafish
|Faucherre A,López-Schier H||PloS one (6:null)||2011|
MYC insertions in diffuse-type gastric adenocarcinoma.
MA5-12077 was used in immunohistochemistry to evaluate the genomic alterations of MYC in diffuse-type gastric adenocarcinoma
|Calcagno DQ,Guimarães AC,Leal MF,Seabra AD,Khayat AS,Pontes TB,Assumpção PP,De Arruda Cardoso Smith M,Burbano RR||Anticancer research (29:2479)||2009|
Prognostic significance of oncogenic markers in ductal carcinoma in situ of the breast: a clinicopathologic study.
MA5-12077 was used in immunohistochemistry to evaluate oncogenic markers with prognostic significance in breast ductal carcinoma in situ
|Altintas S,Lambein K,Huizing MT,Braems G,Asjoe FT,Hellemans H,Van Marck E,Weyler J,Praet M,Van den Broecke R,Vermorken JB,Tjalma WA||The breast journal (15:120)||2009|
Molecular risk stratification of medulloblastoma patients based on immunohistochemical analysis of MYC, LDHB, and CCNB1 expression.
MA5-12077 was used in immunohistochemistry to develop a molecular risk stratification model for medulloblastoma patients
|de Haas T,Hasselt N,Troost D,Caron H,Popovic M,Zadravec-Zaletel L,Grajkowska W,Perek M,Osterheld MC,Ellison D,Baas F,Versteeg R,Kool M||Clinical cancer research : an official journal of the American Association for Cancer Research (14:4154)||2008|
Downregulation of EGF receptor signaling in pancreatic islets causes diabetes due to impaired postnatal beta-cell growth.
MA5-12077 was used in immunohistochemistry and western blot to study the role of EGF receptor signaling in postnatal beta-cell growth in pancreatic islets
|Miettinen PJ,Ustinov J,Ormio P,Gao R,Palgi J,Hakonen E,Juntti-Berggren L,Berggren PO,Otonkoski T||Diabetes (55:3299)||2006|
Expression and prognostic implications of apoptosis-related proteins in locally unresectable non-small cell lung cancers.
MA5-12077 was used in immunohistochemistry to study the prognostic value of apoptosis-related protein expression in locally unresectable non-small cell lung cancers
|Fokkema E,Timens W,de Vries EG,de Jong S,Fidler V,Meijer C,Groen HJ||Lung cancer (Amsterdam, Netherlands) (52:241)||2006|
MYC expression and distribution in normal mature lymphoid cells.
MA5-12077 was used in western blot to study which normal mature lymphoid cells express myc
|Cattoretti G||The Journal of pathology (229:430)||2013|
The dichloromethane extract of the ethnomedicinal plant Neurolaena lobata inhibits NPM/ALK expression which is causal for anaplastic large cell lymphomagenesis.
MA5-12077 was used in western blot to study the role of reduced NPM/ALK expression in the potential therapeutic mechanism of an ethnomedical plant extract against anaplastic large cell lymphoma
|Unger C,Popescu R,Giessrigl B,Laimer D,Heider S,Seelinger M,Diaz R,Wallnöfer B,Egger G,Hassler M,Knöfler M,Saleh L,Sahin E,Grusch M,Fritzer-Szekeres M,Dolznig H,Frisch R,Kenner L,Kopp B,Krupitza G||International journal of oncology (42:338)||2013|
Nonconserved lysine residues attenuate the biological function of the low-risk human papillomavirus E7 protein.
MA5-12077 was used in western blot to study the effect of non-conserved lysine residues on the function of human papillomavirus E7 protein
|Genovese NJ,Broker TR,Chow LT||Journal of virology (85:5546)||2011|
Pro- and anticarcinogenic mechanisms of piceatannol are activated dose dependently in MCF-7 breast cancer cells.
MA5-12077 was used in western blot to investigate the mechanism for the biphasic effect of piceatannol on carcinogenesis
|Vo NT,Madlener S,Bago-Horvath Z,Herbacek I,Stark N,Gridling M,Probst P,Giessrigl B,Bauer S,Vonach C,Saiko P,Grusch M,Szekeres T,Fritzer-Szekeres M,Jäger W,Krupitza G,Soleiman A||Carcinogenesis (31:2074)||2010|
Proteomic analysis reveals Hrs ubiquitin-interacting motif-mediated ubiquitin signaling in multiple cellular processes.
MA5-12077 was used in western blot to study the role of ubiquitin signaling in multiple cellular processes using a proteomic analysis
|Pridgeon JW,Webber EA,Sha D,Li L,Chin LS||The FEBS journal (276:118)||2009|
Mitochondrial p32 is a critical mediator of ARF-induced apoptosis.
MA5-12077 was used in western blot to examine the role of mitochondrial p32 in ARF-induced apoptosis
|Itahana K,Zhang Y||Cancer cell (13:542)||2008|
Defined genetic events associated with the spontaneous in vitro transformation of ElA/Ras-expressing human IMR90 fibroblasts.
MA5-12077 was used in western blot to study the genetic events associated with spontaneous in vitro transformation of EIA/Ras-expressing human fibroblasts
|Mason DX,Keppler D,Zhang J,Jackson TJ,Seger YR,Matsui S,Abreo F,Cowell JK,Hannon GJ,Lowe SW,Lin AW||Carcinogenesis (27:350)||2006|
|Not Applicable||Not Cited||
The shed ectodomain of type XIII collagen associates with the fibrillar fibronectin matrix and may interfere with its assembly in vitro.
MA5-12077 was used in western blot to study the interplay between the shed soluble ectodomain of type XIII collagen and the fibrillar fibronectin matrix
|Väisänen MR,Väisänen T,Tu H,Pirilä P,Sormunen R,Pihlajaniemi T||The Biochemical journal (393:43)||2006|
Sue1p is required for degradation of labile forms of altered cytochromes C in yeast mitochondria.
MA5-12077 was used in western blot to study the role of the nuclear encoded mitochondrial protein Sue1p in the degradation of altered holo-iso-1-cytochrome c isoforms in yeast mitochondria
|Wei J,Sherman F||The Journal of biological chemistry (279:30449)||2004|
Transmembrane peptides as inhibitors of ErbB receptor signaling.
MA5-12077 was used in western blot to investigate the interaction between transmembrane domain of receptor tyrosine kinases and ErbB receptor
|Bennasroune A,Fickova M,Gardin A,Dirrig-Grosch S,Aunis D,Crémel G,Hubert P||Molecular biology of the cell (15:3464)||2004|
Upstream stimulatory factor but not c-Myc enhances transcription of the human polymeric immunoglobulin receptor gene.
MA5-12077 was used in western blot to study an upstream regulatory factor that enhances expression of the human polymeric immunoglobulin receptor
|Bruno ME,West RB,Schneeman TA,Bresnick EH,Kaetzel CS||Molecular immunology (40:695)||2004|
Detection of protein-RNA complexes in Xenopus oocytes.
MA5-12077 was used in immunoprecipitation to develop a method for detecting protein-RNA complexes in Xenopus oocytes
|Huber PW,Zhao WM||Methods (San Diego, Calif.) (51:82)||2010|
Metastasis-associated protein 1 interacts with NRIF3, an estrogen-inducible nuclear receptor coregulator.
MA5-12077 was used in immunoprecipitation to investigate the interaction between MTA1 and NRIF3
|Talukder AH,Gururaj A,Mishra SK,Vadlamudi RK,Kumar R||Molecular and cellular biology (24:6581)||2004|
Construction of tumor-specific cells expressing a membrane-anchored single-chain Fv of anti-ErbB-2 antibody.
MA5-12077 was used in flow cytometry to develop tumor-specific cells expressing a membrane-anchored single-chain Fv of anti-ErbB-2 antibody for drug targeting applications
|Suzuki M,Shinkai M,Honda H,Kamihira M,Iijima S,Kobayashi T||Biochimica et biophysica acta (1525:191)||2001|
Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53.
MA5-12077 was used in immunocytochemistry to study the effect of mutations in exon 2 of human ARF on its nucleolar localization and ability to regulate nuclear export of MDM2 and p53
|Zhang Y,Xiong Y||Molecular cell (3:579)||1999|
ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways.
MA5-12077 was used in immunocytochemistry to investigate the effect of ARF on p53 regulation and its mechanism
|Zhang Y,Xiong Y,Yarbrough WG||Cell (92:725)||1998|