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|Tested species reactivity||Human|
|Published species reactivity||Yeast, Human, Mouse, Not Applicable, Xenopus|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||A synthetic peptide, aa 408-439, (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:50|
|Immunomicroscopy (IM)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 7 publications below|
|Western Blot (WB)||See 11 publications below|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
MA5-12080 detects-myc in Human samples Does not react with mouse, rat and chicken. Others not tested..
MA5-12080 targets c-myc in FACS, IF, IHC (P), IM, IP, and WB applications.
The MA5-12080 immunogen is a synthetic peptide, aa 408-439, (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc.
c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
BCL2, BCL6, IGH, TP53, and MYC protein expression and gene rearrangements as prognostic markers in diffuse large B-cell lymphoma: a study of 44 Turkish patients.
MA5-12080 was used in immunohistochemistry to study the prognostic value of immunohistochemical and FISH analysis of BCL2, BCL6, IGH, TP53, and MYC in Turkish patients with diffuse large B-cell lymphoma
|Akay OM,Aras BD,Isiksoy S,Toprak C,Mutlu FS,Artan S,Oner U,Gulbas Z||Cancer genetics (207:87)||2014|
EGFR and MYC gene copy number aberrations are more common in squamous cell carcinoma than keratoacanthoma: a FISH study.
MA5-12080 was used in immunohistochemistry to use FISH to study the higher frequency of EGFR and MYC gene amplification in squamous cell carcinoma than in keratoacanthoma
|Jacobs MS,Persons DL,Fraga GR||Journal of cutaneous pathology (40:447)||2013|
Expression of p53, Ki-67 and c-Myc proteins is predictive of the surgical molecular margin in colorectal carcinoma.
MA5-12080 was used in immunohistochemistry to study the value of p53, Ki-67 and c-myc expression in determining the surgical margin in colorectal carcinoma resection
|Li N,Wang J,Shen S,Bu X,Tian X,Huang P||Pathology oncology research : POR (17:479)||2011|
MYC insertions in diffuse-type gastric adenocarcinoma.
MA5-12080 was used in immunohistochemistry to evaluate the genomic alterations of MYC in diffuse-type gastric adenocarcinoma
|Calcagno DQ,Guimarães AC,Leal MF,Seabra AD,Khayat AS,Pontes TB,Assumpção PP,De Arruda Cardoso Smith M,Burbano RR||Anticancer research (29:2479)||2009|
Prognostic significance of oncogenic markers in ductal carcinoma in situ of the breast: a clinicopathologic study.
MA5-12080 was used in immunohistochemistry to evaluate oncogenic markers with prognostic significance in breast ductal carcinoma in situ
|Altintas S,Lambein K,Huizing MT,Braems G,Asjoe FT,Hellemans H,Van Marck E,Weyler J,Praet M,Van den Broecke R,Vermorken JB,Tjalma WA||The breast journal (15:120)||2009|
Molecular risk stratification of medulloblastoma patients based on immunohistochemical analysis of MYC, LDHB, and CCNB1 expression.
MA5-12080 was used in immunohistochemistry to develop a molecular risk stratification model for medulloblastoma patients
|de Haas T,Hasselt N,Troost D,Caron H,Popovic M,Zadravec-Zaletel L,Grajkowska W,Perek M,Osterheld MC,Ellison D,Baas F,Versteeg R,Kool M||Clinical cancer research : an official journal of the American Association for Cancer Research (14:4154)||2008|
Expression and prognostic implications of apoptosis-related proteins in locally unresectable non-small cell lung cancers.
MA5-12080 was used in immunohistochemistry to study the prognostic value of apoptosis-related protein expression in locally unresectable non-small cell lung cancers
|Fokkema E,Timens W,de Vries EG,de Jong S,Fidler V,Meijer C,Groen HJ||Lung cancer (Amsterdam, Netherlands) (52:241)||2006|
MYC expression and distribution in normal mature lymphoid cells.
MA5-12080 was used in western blot to study which normal mature lymphoid cells express myc
|Cattoretti G||The Journal of pathology (229:430)||2013|
The dichloromethane extract of the ethnomedicinal plant Neurolaena lobata inhibits NPM/ALK expression which is causal for anaplastic large cell lymphomagenesis.
MA5-12080 was used in western blot to study the role of reduced NPM/ALK expression in the potential therapeutic mechanism of an ethnomedical plant extract against anaplastic large cell lymphoma
|Unger C,Popescu R,Giessrigl B,Laimer D,Heider S,Seelinger M,Diaz R,Wallnöfer B,Egger G,Hassler M,Knöfler M,Saleh L,Sahin E,Grusch M,Fritzer-Szekeres M,Dolznig H,Frisch R,Kenner L,Kopp B,Krupitza G||International journal of oncology (42:338)||2013|
Nonconserved lysine residues attenuate the biological function of the low-risk human papillomavirus E7 protein.
MA5-12080 was used in western blot to study the effect of non-conserved lysine residues on the function of human papillomavirus E7 protein
|Genovese NJ,Broker TR,Chow LT||Journal of virology (85:5546)||2011|
Pro- and anticarcinogenic mechanisms of piceatannol are activated dose dependently in MCF-7 breast cancer cells.
MA5-12080 was used in western blot to investigate the mechanism for the biphasic effect of piceatannol on carcinogenesis
|Vo NT,Madlener S,Bago-Horvath Z,Herbacek I,Stark N,Gridling M,Probst P,Giessrigl B,Bauer S,Vonach C,Saiko P,Grusch M,Szekeres T,Fritzer-Szekeres M,Jäger W,Krupitza G,Soleiman A||Carcinogenesis (31:2074)||2010|
Proteomic analysis reveals Hrs ubiquitin-interacting motif-mediated ubiquitin signaling in multiple cellular processes.
MA5-12080 was used in western blot to study the role of ubiquitin signaling in multiple cellular processes using a proteomic analysis
|Pridgeon JW,Webber EA,Sha D,Li L,Chin LS||The FEBS journal (276:118)||2009|
Mitochondrial p32 is a critical mediator of ARF-induced apoptosis.
MA5-12080 was used in western blot to examine the role of mitochondrial p32 in ARF-induced apoptosis
|Itahana K,Zhang Y||Cancer cell (13:542)||2008|
Defined genetic events associated with the spontaneous in vitro transformation of ElA/Ras-expressing human IMR90 fibroblasts.
MA5-12080 was used in western blot to study the genetic events associated with spontaneous in vitro transformation of EIA/Ras-expressing human fibroblasts
|Mason DX,Keppler D,Zhang J,Jackson TJ,Seger YR,Matsui S,Abreo F,Cowell JK,Hannon GJ,Lowe SW,Lin AW||Carcinogenesis (27:350)||2006|
|Not Applicable||Not Cited||
The shed ectodomain of type XIII collagen associates with the fibrillar fibronectin matrix and may interfere with its assembly in vitro.
MA5-12080 was used in western blot to study the interplay between the shed soluble ectodomain of type XIII collagen and the fibrillar fibronectin matrix
|Väisänen MR,Väisänen T,Tu H,Pirilä P,Sormunen R,Pihlajaniemi T||The Biochemical journal (393:43)||2006|
Sue1p is required for degradation of labile forms of altered cytochromes C in yeast mitochondria.
MA5-12080 was used in western blot to study the role of the nuclear encoded mitochondrial protein Sue1p in the degradation of altered holo-iso-1-cytochrome c isoforms in yeast mitochondria
|Wei J,Sherman F||The Journal of biological chemistry (279:30449)||2004|
Transmembrane peptides as inhibitors of ErbB receptor signaling.
MA5-12080 was used in western blot to investigate the interaction between transmembrane domain of receptor tyrosine kinases and ErbB receptor
|Bennasroune A,Fickova M,Gardin A,Dirrig-Grosch S,Aunis D,Crémel G,Hubert P||Molecular biology of the cell (15:3464)||2004|
Upstream stimulatory factor but not c-Myc enhances transcription of the human polymeric immunoglobulin receptor gene.
MA5-12080 was used in western blot to study an upstream regulatory factor that enhances expression of the human polymeric immunoglobulin receptor
|Bruno ME,West RB,Schneeman TA,Bresnick EH,Kaetzel CS||Molecular immunology (40:695)||2004|
|Not Applicable||Not Cited||
Activation of myeloid cell-specific adhesion class G protein-coupled receptor EMR2 via ligation-induced translocation and interaction of receptor subunits in lipid raft microdomains.
MA5-12080 was used in western blot to demonstrate a critical role for the GPCR proteolysis site in autoproteolysis that mediates receptor signaling and cell activation.
|Huang YS,Chiang NY,Hu CH,Hsiao CC,Cheng KF,Tsai WP,Yona S,Stacey M,Gordon S,Chang GW,Lin HH||Molecular and cellular biology (32:1408)||2012|
Ribosomal protein L11 recruits miR-24/miRISC to repress c-Myc expression in response to ribosomal stress.
MA5-12080 was used in western blot to investigate the relationship between c-Myc and ribosomal biogenesis.
|Challagundla KB,Sun XX,Zhang X,DeVine T,Zhang Q,Sears RC,Dai MS||Molecular and cellular biology (31:4007)||2011|
Detection of protein-RNA complexes in Xenopus oocytes.
MA5-12080 was used in immunoprecipitation to develop a method for detecting protein-RNA complexes in Xenopus oocytes
|Huber PW,Zhao WM||Methods (San Diego, Calif.) (51:82)||2010|
Metastasis-associated protein 1 interacts with NRIF3, an estrogen-inducible nuclear receptor coregulator.
MA5-12080 was used in immunoprecipitation to investigate the interaction between MTA1 and NRIF3
|Talukder AH,Gururaj A,Mishra SK,Vadlamudi RK,Kumar R||Molecular and cellular biology (24:6581)||2004|
|Not Applicable||Not Cited||
Quantitative measurement of anti-ErbB-2 antibody by flow cytometry and ELISA.
MA5-12080 was used in immunocytochemistry and western blot to develop anti-ErbB-2 antibody assays using flow cytometry and ELISA.
|Piechocki MP,Pilon SA,Wei WZ||Journal of immunological methods (259:33)||2002|
Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53.
MA5-12080 was used in immunocytochemistry to study the effect of mutations in exon 2 of human ARF on its nucleolar localization and ability to regulate nuclear export of MDM2 and p53
|Zhang Y,Xiong Y||Molecular cell (3:579)||1999|
ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways.
MA5-12080 was used in immunocytochemistry to investigate the effect of ARF on p53 regulation and its mechanism
|Zhang Y,Xiong Y,Yarbrough WG||Cell (92:725)||1998|
Construction of tumor-specific cells expressing a membrane-anchored single-chain Fv of anti-ErbB-2 antibody.
MA5-12080 was used in flow cytometry to develop tumor-specific cells expressing a membrane-anchored single-chain Fv of anti-ErbB-2 antibody for drug targeting applications
|Suzuki M,Shinkai M,Honda H,Kamihira M,Iijima S,Kobayashi T||Biochimica et biophysica acta (1525:191)||2001|
avian myelocytomatosis viral oncogene homolog; class E basic helix-loop-helix protein 39; myc proto-oncogene protein; myc-related translation/localization regulatory factor; proto-oncogene c-Myc; transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog
BHLHE39; c-Myc; MRTL; MYC; MYCC